Journal Article

Ferritin heavy chain protects the developing wing from reactive oxygen species and ferroptosis

Pascual, Justine, Kolotuev, Irina, Mumbauer, Simone, Hamaratoglu, Fisun

2019

Overview

The interplay between signalling pathways and metabolism is crucial for tissue growth. Yet, it remains poorly understood. Here, we studied the consequences of modulating iron metabolism on the growth of Drosophila imaginal discs. We find that reducing the levels of the ferritin heavy chain in the larval wing discs leads to drastic growth defects, whereas light chain depletion causes only minor defects. Mutant cell clones for the heavy chain lack the ability to compete against Minute mutant cells. Reactive oxygen species (ROS) accumulate in wing discs with reduced heavy chain levels, causing severe mitochondrial defects and ferroptosis. Preventing ROS accumulation alleviates some of the growth defects. We propose that the increased expression of ferritin in hippo mutant cells may protect against ROS accumulation.

• Publisher: Public Library of Science,Public Library of Science (PLoS)
• Subject: Abnormalities / Analysis / Animal wings / Animals / Apoferritins - metabolism / Apoferritins - physiology / Apoptosis / Biology and Life Sciences / Cancer / Cell Death / Cell growth / Cells, Cultured / Defects / Drosophila / Drosophila - genetics / Drosophila - metabolism / Drosophila Proteins - metabolism / Ferritin / Ferroptosis / Ferroptosis - physiology / Genes / Genetic aspects / Genomics / Growth / Homeostasis / Imaginal discs / Imaginal Discs - metabolism / Influence / Insects / Iron - metabolism / Kinases / Light / Metabolism / Microscopy / Mitochondria / Mitochondria - metabolism / Oxidative stress / Paraproteinemias / Prevention / Reactive oxygen species / Reactive Oxygen Species - metabolism / Research and Analysis Methods / Signal Transduction / Wing discs / Wings (Animal) / Wings, Animal - growth & development / Wings, Animal - metabolism