Journal Article

Genetic mapping and physiological analysis of chlorophyll-deficient mutant in Brassica napus L

Lin, Na, Gao, Yumin, Zhou, Qingyuan, Li, Jiana, Liu, Liezhao, Ping, Xiaoke, Yin, Jiaming

2022

Overview

Background Leaf color mutants have reduced photosynthetic efficiency, which has severely negative impacts on crop growth and economic product yield. There are different chlorophyll mutants in Arabidopsis and crops that can be used for genetic control and molecular mechanism studies of chlorophyll biosynthesis, chloroplast development and photoefficiency. Chlorophyll mutants in Brassica napus are mostly used for mapping and location research but are rarely used for physiological research. The chlorophyll-deficient mutant in this experiment were both genetically mapped and physiologically analyzed. Results In this study, yellow leaf mutant of Brassica napus L. mutated by ethyl methyl sulfone (EMS) had significantly lower chlorophyll a, b and carotenoid contents than the wild type, and the net photosynthetic efficiency, stomatal conductance and transpiration rate were all significantly reduced. The mutant had sparse chloroplast distribution and weak autofluorescence. The granule stacks were reduced, and the shape was extremely irregular, with more broken stromal lamella. Transcriptome data analysis enriched the differentially expressed genes mainly in phenylpropane and sugar metabolism. The mutant was mapped to a 2.72 Mb region on A01 by using BSA-Seq, and the region was validated by SSR markers. Conclusions The mutant chlorophyll content and photosynthetic efficiency were significantly reduced compared with those of the wild type. Abnormal chloroplasts and thylakoids less connected to the stroma lamella appeared in the mutant. This work on the mutant will facilitate the process of cloning the BnaA01.cd gene and provide more genetic and physiological information concerning chloroplast development in Brassica napus .

• Publisher: BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
• Subject: Agriculture / Analysis / Arabidopsis / Biomedical and Life Sciences / Biosynthesis / Brassica / Brassica napus / Carbohydrates / Carotenoids / Chlorophyll / Chloroplasts / Chromosome mapping / Chromosomes / Cloning / color / Color of leaves / Crop growth / Data analysis / Deficient mutant / Diseases and pests / Efficiency / gene expression regulation / Gene mapping / Genes / Genetic analysis / Genetic aspects / Genetic control / Genetic mapping / Genotype & phenotype / Growth / Lamella / Leaf color / Leaves / Life Sciences / Metabolism / Metabolites / Methods / Molecular mechanics / Mutant / mutants / Mutation / Photosynthesis / Physiology / Plant Sciences / Rape (Plant) / Rape plants / Rice / Seeds / Stomata / Stomatal conductance / Stroma / sugars / Thylakoids / Transcription factors / Transcriptome / Transcriptomes / Transpiration / Tree Biology / Yellow leaf