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A dispensable SepIVA orthologue in Streptomyces venezuelae is associated with polar growth and not cell division
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A dispensable SepIVA orthologue in Streptomyces venezuelae is associated with polar growth and not cell division
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Journal Article
A dispensable SepIVA orthologue in Streptomyces venezuelae is associated with polar growth and not cell division
2024
Overview
Background
SepIVA has been reported to be an essential septation factor in
Mycolicibacterium smegmatis
and
Mycobacterium tuberculosis
. It is a coiled-coil protein with similarity to DivIVA, a protein necessary for polar growth in members of the phylum Actinomycetota. Orthologues of SepIVA are broadly distributed among actinomycetes, including in
Streptomyces
spp.
Results
To clarify the role of SepIVA and its potential involvement in cell division in streptomycetes, we generated
sepIVA
deletion mutants in
Streptomyces venezuelae
and found that
sepIVA
is dispensable for growth, cell division and sporulation. Further, mNeonGreen-SepIVA fusion protein did not localize at division septa, and we found no evidence of involvement of SepIVA in cell division. Instead, mNeonGreen-SepIVA was accumulated at the tips of growing vegetative hyphae in ways reminiscent of the apical localization of polarisome components like DivIVA. Bacterial two-hybrid system analyses revealed an interaction between SepIVA and DivIVA. The results indicate that SepIVA is associated with polar growth. However, no phenotypic effects of
sepIVA
deletion could be detected, and no evidence was observed of redundancy with the other DivIVA-like coiled-coil proteins Scy and FilP that are also associated with apical growth in streptomycetes.
Conclusions
We conclude that
S. venezuelae
SepIVA, in contrast to the situation in mycobacteria, is dispensable for growth and viability. The results suggest that it is associated with polar growth rather than septum formation.
Publisher
BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
Subject
/ Analysis
/ Bacteria
/ Bacterial Proteins - genetics
/ Bacterial Proteins - metabolism
/ Biologi
/ Biomedical and Life Sciences
/ Cell Cycle Proteins - genetics
/ Cell Cycle Proteins - metabolism
/ Coils
/ Control
/ Deletion
/ E coli
/ FtsZ
/ Genomes
/ Hyphae
/ Identification and classification
/ Mycology
/ Plasmids
/ Proteins
/ Spores, Bacterial - genetics
/ Spores, Bacterial - growth & development
/ Spores, Bacterial - metabolism
/ Streptomyces - growth & development
/ Two-Hybrid System Techniques
/ Virology
