Journal Article

Transcriptome and metabolome analysis revealed the dynamic change of bioactive compounds of Fructus Ligustri Lucidi

Dai, Shilin, Zhou, Peina, Dang, Jingjie, Wu, Qinan, Jiang, Zheng, Qu, Cheng

2024

Overview

Background The Fructus Ligustri Lucidi, the fruit of Ligustrum lucidum , contains a variety of bioactive compounds, such as flavonoids, triterpenoids, and secoiridoids. The proportions of these compounds vary greatly during the different fruit development periods of Fructus Ligustri Lucidi. However, a clear understanding of how the proportions of the compounds and their regulatory biosynthetic mechanisms change across the different fruit development periods of Fructus Ligustri Lucidi is still lacking. Results In this study, metabolite profiling and transcriptome analysis of six fruit development periods (45 DAF, 75 DAF, 112 DAF, 135 DAF, 170 DAF, and 195 DAF) were performed. Seventy compounds were tentatively identified, of which secoiridoids were the most abundant. Eleven identified compounds were quantified by high performance liquid chromatography. A total of 103,058 unigenes were obtained from six periods of Fructus Ligustri Lucidi. Furthermore, candidate genes involved in triterpenoids, phenylethanols, and oleoside-type secoiridoid biosynthesis were identified and analyzed. The in vitro enzyme activities of nine glycosyltransferases involved in salidroside biosynthesis revealed that they can catalyze trysol and hydroxytyrosol to salidroside and hydroxylsalidroside. Conclusions These results provide valuable information to clarify the profile and molecular regulatory mechanisms of metabolite biosynthesis, and also in optimizing the harvest time of this fruit.

• Publisher: BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
• Subject: Agriculture / Analysis / Antifungal agents / Bioactive compounds / Biological activity / Biomedical and Life Sciences / Biosynthesis / Enzymatic activity / Flavonoids / Food additives / Fructus Ligustri Lucidi / Fruit - chemistry / Fruit - genetics / Fruit - metabolism / fruiting / Fruits / Gene Expression Profiling / Gene Expression Regulation, Plant / Genes / glycosyltransferases / harvest date / High performance liquid chromatography / Kinases / Life Sciences / Ligustrum - chemistry / Ligustrum - genetics / Ligustrum - metabolism / Ligustrum lucidum / Liquid chromatography / Metabolism / Metabolites / Metabolome / Metabolomics / Plant Sciences / Quality control / Regulatory mechanisms (biology) / salidroside / Salidroside biosynthesis / Secoiridoid biosynthesis / secoiridoids / Transcriptome / Transcriptomes / transcriptomics / Tree Biology / Triterpenoids / unigenes