Journal Article

Bacterial communities in different sections of a municipal wastewater treatment plant revealed by 16S rDNA 454 pyrosequencing

Zhang, Tong, Ye, Lin

2013

Overview

In this study, we successfully demonstrated that 454 pyrosequencing was a powerful approach for investigating the bacterial communities in the activated sludge, digestion sludge, influent, and effluent samples of a full scale wastewater treatment plant treating saline sewage. For each sample, 18,808 effective sequences were selected and utilized to do the bacterial diversity and abundance analysis. In total, 2,455, 794, 1,667, and 1,932 operational taxonomic units were obtained at 3 % distance cutoff in the activated sludge, digestion sludge, influent, and effluent samples, respectively. The corresponding most dominant classes in the four samples are Alphaproteobacteria , Thermotogae , Deltaproteobacteria , and Gammaproteobacteria . About 67 % sequences in the digestion sludge sample were found to be affiliated with the Thermotogales order. Also, these sequences were assigned into a recently proposed genus Kosmotoga by the Ribosomal Database Project classifier . In the effluent sample, we found high abundance of Mycobacterium and Vibrio , which are genera containing pathogenic bacteria. Moreover, in this study, we proposed a method to differentiate the “gene percentage” and “cell percentage” by using Ribosomal RNA Operon Copy Number Database.

• Publisher: Springer-Verlag,Springer,Springer Nature B.V
• Subject: Activated sludge / alpha-Proteobacteria / Bacteria / Bacteria - classification / Bacteria - genetics / bacterial communities / Bacterial genetics / Bar codes / Biomedical and Life Sciences / Biota / Biotechnology / Cell differentiation / chemistry / classification / Cluster Analysis / Communities / Copy number / delta-Proteobacteria / Digestion / DNA sequencing / DNA, Bacterial / DNA, Bacterial - chemistry / DNA, Bacterial - genetics / DNA, Ribosomal / DNA, Ribosomal - chemistry / DNA, Ribosomal - genetics / Effluents / Environmental aspects / Environmental Biotechnology / Environmental monitoring / Genera / Genetic testing / genetics / Influents / Laboratories / Life Sciences / Metagenome / Microbial Genetics and Genomics / Microbiology / Molecular Sequence Data / Municipal wastewater / Mycobacterium / Nucleotide sequencing / operon / Phylogeny / ribosomal DNA / ribosomal RNA / RNA, Ribosomal, 16S / RNA, Ribosomal, 16S - genetics / rRNA / rRNA 16S / Sequence Analysis, DNA / Sequences / Sewage / Sewage disposal plants / Sludge / Sludge digestion / Studies / Thermotogales / Vibrio / Wastewater treatment / Wastewater treatment plants / Water Microbiology / Water pollution / Water Purification / Water treatment