Asset Details
Do you wish to reserve the book?
Identification of the new allele ptc1-2 and analysis of the regulatory role of PTC1 gene in rice anther development
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Identification of the new allele ptc1-2 and analysis of the regulatory role of PTC1 gene in rice anther development
Do you wish to request the book?
Identification of the new allele ptc1-2 and analysis of the regulatory role of PTC1 gene in rice anther development
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Identification of the new allele ptc1-2 and analysis of the regulatory role of PTC1 gene in rice anther development
2024
Overview
Anther development involves a series of important biological events that are precisely regulated by many genes. Although several important genes involved in rice anther development have been identified, the regulatory network involved in tapetal development and pollen wall formation is still largely unclear.
PERSISTENT TAPETAL CELL 1
(
PTC1
) encodes a PHD-Finger protein, which plays a critical role in the regulation of tapetal cell death and pollen development in rice. Here, we report the isolation and characterization of a new allele
ptc1-2
with 2-base deletion in the third exon, causing the absent of the PHD domain due to the sequence change. Cytological analysis revealed delayed tapetal PCD, defective pollen exine formation and abnormal ubisch bodies development. Transcriptome analysis revealed that genes related to pollen wall formation (secondary metabolism, phenylalanine synthesis, and cutin and wax biosynthesis pathways), cell death (cysteine and methionine metabolism and DNA repair pathways), and carbohydrate synthesis (starch and sucrose metabolism pathways) were significantly altered in
ptc1-2
mutant. A total of 13 reported anther development genes exhibited significant expression changes in the
ptc1-2
mutant. Yeast two-hybrid and BiFC analyses showed that PTC1 could interact with API5, an inhibitor of apoptosis, and the citrin-binding enzyme EDT1. This work is helpful in deepening the understanding of the regulatory network of male reproductive development in rice.
Publisher
BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
Subject
/ Alleles
/ Analysis
/ Anther
/ API5
/ Biomedical and Life Sciences
/ Cloning
/ Cutin
/ cysteine
/ domain
/ EDT1
/ Enzymes
/ exine
/ exons
/ Flowers - growth & development
/ Gene Expression Regulation, Plant
/ Genes
/ Genomics
/ males
/ Mutants
/ Oryza - growth & development
/ Plants
/ Pollen
/ Pollen - growth & development
/ Proteins
/ ptc1-2
/ Rice
/ starch
/ Sucrose
/ Tapetum
/ two hybrid system techniques
/ Yeast
