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RpoS integrates CRP, Fis, and PhoP signaling pathways to control Salmonella Typhi hlyE expression
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RpoS integrates CRP, Fis, and PhoP signaling pathways to control Salmonella Typhi hlyE expression
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Journal Article
RpoS integrates CRP, Fis, and PhoP signaling pathways to control Salmonella Typhi hlyE expression
2014
Overview
Background
SPI-18 is a pathogenicity island found in some
Salmonella enterica
serovars, including
S.
Typhi. SPI-18 harbors two ORFs organized into an operon,
hlyE
and
taiA
genes, both implicated in virulence. Regarding the
hlyE
regulation in
S.
Typhi, it has been reported that RpoS participates as transcriptional up-regulator under low pH and high osmolarity. In addition, CRP down-regulates
hlyE
expression during exponential growth. Previously, it has been suggested that there is another factor related to catabolite repression, different from CRP, involved in the down-regulation of
hlyE
. Moreover, PhoP-dependent
hlyE
up-regulation has been reported in bacteria cultured simultaneously under low pH and low concentration of Mg
2+
. Nevertheless, the relative contribution of each environmental signal is not completely clear. In this work we aimed to better understand the regulation of
hlyE
in
S.
Typhi and the integration of different environmental signals through global regulators.
Results
We found that Fis participates as a CRP-independent glucose-dependent down-regulator of
hlyE
. Also, Fis and CRP seem to exert the repression over
hlyE
through down-regulating
rpoS
. Moreover, PhoP up-regulates
hlyE
expression via
rpoS
under low pH and low Mg
2+
conditions.
Conclusions
All these results together show that, at least under the tested conditions, RpoS is the central regulator in the
hlyE
regulatory network, integrating multiple environmental signals and global regulators.
Publisher
BioMed Central,BioMed Central Ltd,Springer Nature B.V
Subject
Bacterial Proteins - metabolism
/ Biomedical and Life Sciences
/ Cyclic AMP Receptor Protein - metabolism
/ E coli
/ Factor For Inversion Stimulation Protein - metabolism
/ Gene Expression Regulation, Bacterial
/ Genes
/ Glucose
/ Grants
/ Hemolysin Proteins - biosynthesis
/ Mycology
/ Salmonella typhi - metabolism
/ Salmonella typhi - physiology
/ Virology
