Asset Details
Do you wish to reserve the book?
Stable transgene expression and CRISPR-mediated knock-in system of a bacteria-derived antibiotic selection gene in the green alga Ulva prolifera
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Stable transgene expression and CRISPR-mediated knock-in system of a bacteria-derived antibiotic selection gene in the green alga Ulva prolifera
Do you wish to request the book?
Stable transgene expression and CRISPR-mediated knock-in system of a bacteria-derived antibiotic selection gene in the green alga Ulva prolifera
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Stable transgene expression and CRISPR-mediated knock-in system of a bacteria-derived antibiotic selection gene in the green alga Ulva prolifera
2025
Overview
Ulva prolifera
is a fast-growing green seaweed that has garnered considerable interest in both fundamental and applied research. Here, we established a molecular tool by employing a selectable marker gene that allowed the isolation of
U. prolifera
cells integrating exogenous DNA. We developed a modular plasmid for expressing exogenous genes in
U. prolifera
based on the bacterial antibiotic-resistance marker, aminoglycoside phosphotransferase gene (
aph7”
). Integration of
aph7”
in macroalgae can generate transformants resistant to hygromycin B. In addition, we characterized the promoter region of the small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase gene (pUpRbcS) to drive the expression of
aph7”
. The transcripts were consistently confirmed from antibiotic-selected transformants, stably retaining the exogenous gene in the succeeding generations. Subsequently, a CRISPR-based knock-in system was established, facilitating the integration of
aph7”
cassette in the endogenous selection gene encoding for adenine phosphoribosyltransferase (
UpAPT
).
APT
gene can serve as an endogenous marker in algae that exhibits a lethal phenotype under cultivation with 2-fluoroadenine. The resulting knock-in mutants could resist the co-selection of the antibiotic hygromycin B and 2-fluoroadenine. Our results advance
U. prolifera
as a genetic platform, enabling functional research to elucidate
Ulva
biology, and to bring forth biotechnological utilization of algal resources.
Publisher
BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
We currently cannot retrieve any items related to this title. Kindly check back at a later time.