Journal Article

Giant Hydrogen Sulfide Plume in the Oxygen Minimum Zone off Peru Supports Chemolithoautotrophy

Schilhabel, Markus B., Lavik, Gaute, Löscher, Carolin R., Desai, Dhwani K., Schmitz, Ruth A., Schunck, Harald, Kalvelage, Tim, Holtappels, Moritz, Siegel, Herbert, LaRoche, Julie, Contreras, Sergio, Rosenstiel, Philip, Kuypers, Marcel M. M., Graco, Michelle, Paulmier, Aurélien, Großkopf, Tobias

2013

Overview

In Eastern Boundary Upwelling Systems nutrient-rich waters are transported to the ocean surface, fuelling high photoautotrophic primary production. Subsequent heterotrophic decomposition of the produced biomass increases the oxygen-depletion at intermediate water depths, which can result in the formation of oxygen minimum zones (OMZ). OMZs can sporadically accumulate hydrogen sulfide (H2S), which is toxic to most multicellular organisms and has been implicated in massive fish kills. During a cruise to the OMZ off Peru in January 2009 we found a sulfidic plume in continental shelf waters, covering an area >5500 km(2), which contained ∼2.2×10(4) tons of H2S. This was the first time that H2S was measured in the Peruvian OMZ and with ∼440 km(3) the largest plume ever reported for oceanic waters. We assessed the phylogenetic and functional diversity of the inhabiting microbial community by high-throughput sequencing of DNA and RNA, while its metabolic activity was determined with rate measurements of carbon fixation and nitrogen transformation processes. The waters were dominated by several distinct γ-, δ- and ε-proteobacterial taxa associated with either sulfur oxidation or sulfate reduction. Our results suggest that these chemolithoautotrophic bacteria utilized several oxidants (oxygen, nitrate, nitrite, nitric oxide and nitrous oxide) to detoxify the sulfidic waters well below the oxic surface. The chemolithoautotrophic activity at our sampling site led to high rates of dark carbon fixation. Assuming that these chemolithoautotrophic rates were maintained throughout the sulfidic waters, they could be representing as much as ∼30% of the photoautotrophic carbon fixation. Postulated changes such as eutrophication and global warming, which lead to an expansion and intensification of OMZs, might also increase the frequency of sulfidic waters. We suggest that the chemolithoautotrophically fixed carbon may be involved in a negative feedback loop that could fuel further sulfate reduction and potentially stabilize the sulfidic OMZ waters.

• Publisher: Public Library of Science,Public Library of Science (PLoS)
• Subject: Anesthetics / Bacteria / Bacteria - genetics / Bacteria - isolation & purification / Bacteria - metabolism / Biogeochemistry / Bioinformatics / Biology / Biomass / Carbon / Carbon Cycle / Carbon Dioxide - chemistry / Carbon fixation / Chemistry / Chemoautotrophic Growth - physiology / Climate change / Cluster Analysis / Colloids - chemistry / Continental shelves / Deoxyribonucleic acid / DNA / DNA sequencing / Ecosystem / Eutrophication / Feedback loops / Fish kills / Flow Cytometry - methods / Gene sequencing / Genome, Bacterial / Genomes / Global temperature changes / Global warming / Hydrogen / Hydrogen sulfide / Hydrogen Sulfide - chemistry / Hypotheses / Intermediate water / Microorganisms / Molecular biology / Negative feedback / Nitrates / Nitric oxide / Nitrogen - chemistry / Nitrous oxide / Ocean surface / Ocean, Atmosphere / Oceanography / Oxidants / Oxidation / Oxidizing agents / Oxygen / Oxygen - chemistry / Oxygen depletion / Pacific Ocean / Peru / Phylogeny / Primary production / Reduction / Ribonucleic acid / RNA / Sciences of the Universe / Seawater - chemistry / Sediments / Sequence Analysis, DNA / Sequence Analysis, RNA / Studies / Sulfate reduction / Sulfates / Sulfide / Sulfides / Sulfides - chemistry / Sulfur / Sulfur oxidation / Taxa / Transformation / Upwelling / Water depth / Water Microbiology