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PcDWF1, a pear brassinosteroid biosynthetic gene homologous to AtDWARF1, affected the vegetative and reproductive growth of plants
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PcDWF1, a pear brassinosteroid biosynthetic gene homologous to AtDWARF1, affected the vegetative and reproductive growth of plants
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Journal Article
PcDWF1, a pear brassinosteroid biosynthetic gene homologous to AtDWARF1, affected the vegetative and reproductive growth of plants
2020
Overview
Background
The steroidal hormones brassinosteroids (BRs) play important roles in plant growth and development. The pathway and genes involved in BR biosynthesis have been identified primarily in model plants like
Arabidopsis
, but little is known about BR biosynthesis in woody fruits such as pear.
Results
In this study, we found that applying exogenous brassinolide (BL) could significantly increase the stem growth and rooting ability of
Pyrus ussuriensis
.
PcDWF1
, which had a significantly lower level of expression in the dwarf-type pear than in the standard-type pear, was cloned for further analysis. A phylogenetic analysis showed that
PcDWF1
was a pear brassinosteroid biosynthetic gene that was homologous to
AtDWARF1
. The subcellular localization analysis indicated that PcDWF1 was located in the plasma membrane. Overexpression of
PcDWF1
in tobacco (
Nicotiana tabacum
) or pear (
Pyrus ussuriensis
) plants promoted the growth of the stems, which was caused by a larger cell size and more developed xylem than those in the control plants, and the rooting ability was significantly enhanced. In addition to the change in vegetative growth, the tobacco plants overexpressing
PcDWF1
also had a delayed flowering time and larger seed size than did the control tobacco plants. These phenotypes were considered to result from the higher BL contents in the transgenic lines than in the control tobacco and pear plants.
Conclusions
Taken together, these results reveal that the pear BR biosynthetic gene
PcDWF1
affected the vegetative and reproductive growth of
Pyrus ussuriensis
and
Nicotiana tabacum
and could be characterized as an important BR biosynthetic gene in perennial woody fruit plants.
Publisher
BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
Subject
/ Biomedical and Life Sciences
/ Brassinosteroids - biosynthesis
/ Cloning
/ Development and cell biology
/ Dwarfism
/ Flowers - growth & development
/ Fruit - growth & development
/ Fruits
/ Gene Expression Regulation, Plant
/ Genes
/ genetically modified organisms
/ Growth
/ Homology
/ Hormones
/ Kinases
/ Nicotiana - growth & development
/ PcDWF1
/ Pears
/ Plants, Genetically Modified - genetics
/ Plants, Genetically Modified - growth & development
/ Plasma
/ Pyrus - growth & development
/ Rooting
/ Seeds
/ stems
/ Time
/ Tobacco
/ Vegetative and reproductive growth
/ Xylem
