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Development of Gateway Binary Vector Series with Four Different Selection Markers for the Liverwort Marchantia polymorpha
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Development of Gateway Binary Vector Series with Four Different Selection Markers for the Liverwort Marchantia polymorpha
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Development of Gateway Binary Vector Series with Four Different Selection Markers for the Liverwort Marchantia polymorpha
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Journal Article
Development of Gateway Binary Vector Series with Four Different Selection Markers for the Liverwort Marchantia polymorpha
2015
Overview
We previously reported Agrobacterium-mediated transformation methods for the liverwort Marchantia polymorpha using the hygromycin phosphotransferase gene as a marker for selection with hygromycin. In this study, we developed three additional markers for M. polymorpha transformation: the gentamicin 3'-acetyltransferase gene for selection with gentamicin; a mutated acetolactate synthase gene for selection with chlorsulfuron; and the neomycin phosphotransferase II gene for selection with G418. Based on these four marker genes, we have constructed a series of Gateway binary vectors designed for transgenic experiments on M. polymorpha. The 35S promoter from cauliflower mosaic virus and endogenous promoters for constitutive and heat-inducible expression were used to create these vectors. The reporters and tags used were Citrine, 3×Citrine, Citrine-NLS, TagRFP, tdTomato, tdTomato-NLS, GR, SRDX, SRDX-GR, GUS, ELuc(PEST), and 3×FLAG. These vectors, designated as the pMpGWB series, will facilitate molecular genetic analyses of the emerging model plant M. polymorpha.
Publisher
Public Library of Science,Public Library of Science (PLoS)
Subject
/ Acetolactate Synthase - genetics
/ Acetolactate Synthase - metabolism
/ Acetyltransferases - genetics
/ Acetyltransferases - metabolism
/ Agrobacterium tumefaciens - enzymology
/ Agrobacterium tumefaciens - genetics
/ Analysis
/ Bacterial Proteins - genetics
/ Bacterial Proteins - metabolism
/ Cloning
/ Enzymes
/ Genomes
/ Hygromycin phosphotransferase
/ Kanamycin Kinase - metabolism
/ Marchantia - growth & development
/ Markers
/ Methods
/ Mutation
/ Neomycin
/ Plants, Genetically Modified - growth & development
/ Plasmids
/ Viruses
