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Genome-wide identification and expression analysis of CBF/DREB1 gene family in Medicago sativa L. and functional verification of MsCBF9 affecting flowering time
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Genome-wide identification and expression analysis of CBF/DREB1 gene family in Medicago sativa L. and functional verification of MsCBF9 affecting flowering time
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Genome-wide identification and expression analysis of CBF/DREB1 gene family in Medicago sativa L. and functional verification of MsCBF9 affecting flowering time
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Journal Article
Genome-wide identification and expression analysis of CBF/DREB1 gene family in Medicago sativa L. and functional verification of MsCBF9 affecting flowering time
2025
Overview
Background
The C-repeat binding factor (CBF)/dehydration-responsive element binding (DREB1) belongs to a subfamily of the AP2/ERF (APETALA2/ethylene-responsive factor) superfamily, which can regulate many physiological and biochemical processes in plants, such as plant growth and development, hormone signal transduction and response to abiotic stress. Although the
CBF/DREB1
family has been identified in many plants, studies of the
CBF/DREB1
family in alfalfa are insufficient.
Results
In this study, 25
MsCBF
genes were identified in the genome of alfalfa (“Zhongmu No. 4”). These genes were distributed on chromosomes 1, 5, 6 and unassembled scaffolds. Phylogenetics divided the
CBF
members of
Medicago sativa
,
Arabidopsis thaliana
, and
Medicago truncatula
into six groups, of which group VI had the most
MsCBFs
members, reaching 52% (13/25). Gene duplication analysis showed that 64% (16/25) of
MsCBFs
formed tandem duplications, and 32% (8/25) formed segment duplications. The expression pattern of
MsCBF9
under different hormone treatments was verified by RT-qPCR, and it was found that
MsCBF9
responded to GA3, IAA, SA, and MeJA. Overexpression of
MsCBF9
in
Arabidopsis
significantly delayed the flowering time of
Arabidopsis
. In contrast, the flowering time of
the cbfs
mutant was earlier, and overexpression of
MsCBF9
also increased the number and size of
Arabidopsis
rosette leaves.
Conclusion
In this study, the
CBF/DREB1
family of alfalfa was comprehensively identified and analyzed, and the function of
MsCBF9
in regulating flowering time was studied. This study laid a foundation for further analysis of the function of the
CBF
family in alfalfa.
Clinical trial number
Not applicable.
Publisher
BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
Subject
/ Alfalfa
/ Binding
/ Biomedical and Life Sciences
/ family
/ Flowers - growth & development
/ Gene Expression Regulation, Plant
/ Genes
/ Genomes
/ Identification and classification
/ Medicago sativa - growth & development
/ Medicago sativa - physiology
/ mutants
/ Proteins
/ Rosette
/ Transcription Factors - genetics
