Journal Article

HAWAIIAN SKIRT controls size and floral organ number by modulating CUC1 and CUC2 expression

Szecsi, Judit, Roberts, Jeremy A, Reproduction et développement des plantes (RDP) ; École normale supérieure de Lyon (ENS de Lyon) ; Université de Lyon-Université de Lyon-Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL) ; Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Division of Plant and Crop Sciences, School of Biosciences ; University of Nottingham, UK (UON), University of Nottingham; Radboud University Nijmegen; French "Agence Nationale de la Recherche" ANR-09-BLAN-0006 ANR13-BSV7-0014, ANR-13-BSV7-0014,DODO,Identification et caractérisation des mutations DOMINANT DOUBLE (DODO) and DOUBLE FLOWER (DF) chez le pétunia et la rose, Peters, Janny L, Brookhaven National Laboratory [Upton, NY] (BNL) ; UT-Battelle, LLC-Stony Brook University [SUNY] (SBU) ; State University of New York (SUNY)-State University of New York (SUNY)-U.S. Department of Energy [Washington] (DOE), Plymouth University, Boltz, Véronique, Radboud University [Nijmegen], G

2017

Overview

The Arabidopsis thaliana F-box gene HAWAIIAN SKIRT (HWS) affects organ growth and the timing of floral organ abscission. The loss-of-function hws-1 mutant exhibits fused sepals and increased organ size. To understand the molecular mechanisms of HWS during plant development, we mutagenized hws-1 seeds with ethylmethylsulphonate (EMS) and screened for mutations suppressing hws-1 associated phenotypes. We isolated the shs1/ hws-1 (suppressor of hws-1) mutant in which hws-1 sepal fusion phenotype was suppressed. The shs1/hws-1 mutant carries a G -> A nucleotide substitution in the MIR164 binding site of CUP-SHAPED COTYLEDON 1 (CUC1) mRNA. CUC1 and CUP-SHAPED COTYLEDON 2 (CUC2) transcript levels were altered in shs1, renamed cuc1-1D, and in hws-1 mutant. Genetic interaction analyses using single, double and triple mutants of cuc1-1D, cuc2-1D (a CUC2 mutant similar to cuc1-1D), and hws-1, demonstrate that HWS, CUC1 and CUC2 act together to control floral organ number. Loss of function of HWS is associated with larger petal size due to alterations in cell proliferation and mitotic growth, a role shared with the CUC1 gene.

• Publisher: CCSD,Public Library of Science,Public Library of Science (PLoS)
• Subject: Abscission / Arabidopsis / Arabidopsis - anatomy & histology / Arabidopsis - genetics / Arabidopsis - growth & development / Arabidopsis - metabolism / Arabidopsis Proteins - genetics / Arabidopsis Proteins - metabolism / Arabidopsis thaliana / Binding Sites / Biology and Life Sciences / Boundaries / Cell cycle / Cell division / Cell growth / Cell proliferation / Cell Proliferation - physiology / Cell Size / Crop science / CUC1 gene / F-Box Proteins - genetics / F-Box Proteins - metabolism / Feedback, Physiological - physiology / Flowers & plants / Flowers - anatomy & histology / Flowers - genetics / Flowers - growth & development / Flowers - metabolism / Gene expression / Gene Expression Regulation, Plant / Gene mutation / Genes / Genetic aspects / Life Sciences / MicroRNAs - metabolism / Molecular modelling / Mutagens / Mutants / Mutation / Organ Size / Phenotype / Physiological aspects / Plant flowering / Plants, Genetically Modified / Protein Binding / Proteins / Regulation / Research and Analysis Methods / RNA, Messenger - metabolism / Seeds / Seeds - drug effects / Seeds - genetics / Seeds - growth & development / Seeds - metabolism / Sepals / Transcription / Vegetal Biology
• ISBN: 0004113399000