Journal Article

Resistin in dairy cows: Plasma concentrations during early lactation, expression and potential role in adipose tissue

Dupont, Joëlle, Elis, Sébastien, Briant, Eric, Physiologie de la reproduction et des comportements [Nouzilly] (PRC) ; Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur] (IFCE)-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique; Region Centre "Adipofertikines proposal, Reverchon, Maxime, Cognie, Juliette, Unité Expérimentale de Physiologie Animale de l‘Orfrasiére (UE PAO) ; Institut National de la Recherche Agronomique (INRA), Guillaume, Daniel, Rame, Christelle

2014

Overview

Resistin is an adipokine that has been implicated in energy metabolism regulation in rodents but has been little studied in dairy cows. We determined plasma resistin concentrations in early lactation in dairy cows and investigated the levels of resistin mRNA and protein in adipose tissue and the phosphorylation of several components of insulin signaling pathways one week post partum (1 WPP) and at five months of gestation (5 MG). We detected resistin in mature bovine adipocytes and investigated the effect of recombinant bovine resistin on lipolysis in bovine adipose tissue explants. ELISA showed that plasma resistin concentration was low before calving, subsequently increasing and reaching a peak at 1 WPP, decreasing steadily thereafter to reach pre-calving levels at 6 WPP. Plasma resistin concentration was significantly positively correlated with plasma non esterified fatty acid (NEFA) levels and negatively with milk yield, dry matter intake and energy balance between WPP1 to WPP22. We showed, by quantitative RT-PCR and western blotting, that resistin mRNA and protein levels in adipose tissue were higher at WPP1 than at 5 MG. The level of phosphorylation of several early and downstream insulin signaling components (IRβ, IRS-1, IRS-2, Akt, MAPK ERK1/2, P70S6K and S6) in adipose tissue was also lower at 1 WPP than at 5 MG. Finally, we showed that recombinant bovine resistin increased the release of glycerol and mRNA levels for ATGL (adipose triglyceride lipase) and HSL (hormone-sensitive lipase) in adipose tissue explants. Overall, resistin levels were high in the plasma and adipose tissue and were positively correlated with NEFA levels after calving. Resistin is expressed in bovine mature adipocytes and promotes lipid mobilization in adipose explants in vitro.

• Publisher: Public Library of Science,CCSD,Public Library of Science (PLoS)
• Subject: Adipocytes / Adipocytes - metabolism / Adiponectin - metabolism / Adipose tissue / Adipose Tissue - metabolism / AKT protein / Amino acids / Animal lactation / Animals / Biology and life sciences / Body fat / Cattle / Computer Science / Dairy cattle / Dry matter / Energy balance / Energy law / Energy metabolism / Enzyme-linked immunosorbent assay / Esterification / Explants / Fatty acids / Female / Gene Expression / Gestation / Glycerol / Hormones / Insulin / Insulin receptor substrate 1 / Insulin Receptor Substrate Proteins - metabolism / Insulin resistance / Insulin-like growth factors / Kinases / Lactation / Lactation - blood / Lactation - genetics / Life Sciences / Lipase / Lipase - genetics / Lipase - metabolism / Lipolysis / MAP kinase / MAP Kinase Signaling System / Medicine and Health Sciences / Metabolism / Milk / mRNA / Phosphorylation / Polymerase chain reaction / Postpartum Period / Proteins / Proto-Oncogene Proteins c-akt / Receptor, IGF Type 1 - metabolism / Receptor, Insulin - metabolism / Resistin - blood / Resistin - genetics / Resistin - metabolism / Ribosomal Protein S6 Kinases - metabolism / RNA / RNA, Messenger - genetics / RNA, Messenger - metabolism / Rodents / Signaling / Western blotting
• ISBN: 0003336775000