Journal Article

The Predicted Structure of Immunoglobulin D1.3 and its Comparison with the Crystal Structure

Levitt, Michael, Amit, Adolfo G., Mariuzza, Roy A., Poljak, Roberto J., Lesk, Arthur M., Simon E. V. Phillips, Chothia, Cyrus

1986

Overview

Predictions of the structures of the antigen-binding domains of an antibody, recorded before its experimental structure determination and tested subsequently, were based on comparative analysis of known antibody structures or on conformational energy calculations. The framework, the relative positions of the hypervariable regions, and the folds of four of the hypervariable loops were predicted correctly. This portion includes all residues in contact with the antigen, in this case hen egg white lysozyme, implying that the main chain conformation of the antibody combining site does not change upon ligation. The conformations of three residues in each of the other two hypervariable loops are different in the predicted models and the experimental structure.

• Publisher: The American Association for the Advancement of Science,American Association for the Advancement of Science
• Subject: Amino Acid Sequence / Animals / Antibodies, immunoglobulins / Antibodies, Monoclonal / Antigen-Antibody Complex / Antigen-antibody reactions / Antigens / Atoms / Atoms & subatomic particles / Binding sites / Biological and medical sciences / Chemistry / Chickens / Crystal structure / Egg White / Electron density / Electronic structure / Female / Fundamental and applied biological sciences. Psychology / Fundamental immunology / Hydrogen bonds / immunoglobulin D / Immunoglobulin Fab Fragments / Immunoglobulin G / Immunoglobulin Heavy Chains / Immunoglobulin Light Chains / Immunoglobulin Variable Region / Immunoglobulins / Modeling / Models, Molecular / Molecular immunology / Molecular structure / Muramidase - immunology / Protein Conformation / Structure / Terraces