Journal Article

Immunogenicity of heparin-binding hemagglutinin expressed by Pichia pastoris GS115 strain

Zhu, Ke, Xu, Hefei, Teng, Xindong, Chen, Xiaoguang

2018

Overview

Heparin-binding hemagglutinin (HBHA), a mycobacterial cell surface protein, mediates adhesion to nonphagocytic cells and the dissemination of ( ) from the site of primary infection. Superior expression systems are required to obtain abundant proteins for the purpose of diagnosing infection or for the immunization. Here, HBHA was expressed by ( ) GS115 strain , and the immunogenicity of HBHA was evaluated. The gene of was cloned into the pPIC9K plasmid, which was good for electroporation into GS115 strain. Unlabeled HBHA protein was purified using a Sepharose CL-6B column, and its expression was confirmed using anti-HBHA polyclonal antibody from mouse serum. We injected C57BL/6 mice with HBHA/ dimethyldioctadecylammonium/trehalose 6,6'-dibehenate (HBHA/DDA/TDB) to investigate the immunogenicity of this potential vaccine. The results demonstrated that HBHA/DDA/TDB has the ability to induce high levels of HBHA-specific IgG antibody and its subclasses, as well as interferon-gamma, compared with injection of phosphate-buffered saline, DDA/TDB alone and Bacillus Calmette-Guérin (BCG) controls ( <0.05). Moreover, the ratio of IgG2a/IgG1 of the HBHA/DDA/TDB group was higher than that of the BCG group ( <0.05). HBHA with no label has excellent immunogenicity, and is suitable for evaluating the effectiveness to prevent infection.

• Publisher: Mashhad University of Medical Sciences
• Subject: Anticoagulants / DDA / Heparin-binding- hemagglutinin / Infections / Mycobacterium / Original / Pichia pastoris GS115 / TDB / Tuberculosis