Asset Details
Do you wish to reserve the book?
Natural noncanonical protein splicing yields products with diverse b-amino acid residues
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Natural noncanonical protein splicing yields products with diverse b-amino acid residues
Do you wish to request the book?
Natural noncanonical protein splicing yields products with diverse b-amino acid residues
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Natural noncanonical protein splicing yields products with diverse b-amino acid residues
2018
Overview
Current textbook knowledge holds that the structural scope of ribosomal biosynthesis is based exclusively on a-amino acid backbone topology. Here we report the genome-guided discovery of bacterial pathways that posttranslationally create b-amino acid-containing products. The transformation is widespread in bacteria and is catalyzed by an enzyme belonging to a previously uncharacterized radical S-adenosylmethionine family. We show that the b-amino acids result from an unusual protein splicing process involving backbone carbon-carbon bond cleavage and net excision of tyramine. The reaction can be used to incorporate diverse and multiple b-amino acids into genetically encoded precursors in Escherichia coli. In addition to enlarging the set of basic amino acid components, the excision generates keto functions that are useful as orthogonal reaction sites for chemical diversification.
Publisher
American Association for the Advancement of Science (AAAS)
