Asset Details
Do you wish to reserve the book?
Profile of gene expression changes during estrodiol-17beta-induced feminization in the Takifugu rubripes brain
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Profile of gene expression changes during estrodiol-17beta-induced feminization in the Takifugu rubripes brain
Do you wish to request the book?
Profile of gene expression changes during estrodiol-17beta-induced feminization in the Takifugu rubripes brain
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Profile of gene expression changes during estrodiol-17beta-induced feminization in the Takifugu rubripes brain
2021
Overview
As the critical tissue of the central nervous system, the brain has been found to be involved in gonad development. Previous studies have suggested that gonadal fate may be affected by the brain. Identifying brain-specific molecular changes that occur during estrodiol-17[beta] (E.sub.2) -induced feminization is crucial to our understanding of the molecular control of sex differentiation by the brains of fish. In this study, the differential transcriptomic responses of the Takifugu rubripes larvae brain were compared after E.sub.2 treatment for 55 days. Our results showed that 514 genes were differentially expressed between E.sub.2-treated-XX (E-XX) and Control-XX (C-XX) T. rubripes, while 362 genes were differentially expressed between E.sub.2-treated-XY (E-XY) and Control-XY (C-XY). For example, the expression of cyp19a1b, gnrh1 and pgr was significantly up-regulated, while st, sl, tsh[beta], prl and pit-1, which belong to the growth hormone/prolactin family, were significantly down-regulated after E.sub.2 treatment, in both sexes. The arntl1, bhlbe, nr1d2, per1b, per3, cry1, cipc and ciart genes, which are involved in the circadian rhythm, were also found to be altered. Differentially expressed genes (DEGs), which were identified between E-XX and C-XX, were significantly enriched in neuroactive ligand-receptor interaction, arachidonic acid metabolism, cytokine-cytokine receptor interaction and the calcium signaling pathway. The DEGs that were identified between E-XY and C-XY were significantly enriched in tyrosine metabolism, phenylalanine metabolism, arachidonic acid metabolism and linoleic acid metabolism. A number of genes and pathways were identified in the brain of E.sub.2-treated T. rubripes larvae by RNA-seq. It provided the opportunity for further study on the possible involvement of networks in the brain-pituitary-gonadal axis in sex differentiation in T. rubripes.
Publisher
BioMed Central Ltd
Subject
