Journal Article

Phylogenetic ctDNA analysis depicts early-stage lung cancer evolution

Wilson, Gareth A., Rosenthal, Rachel, Ahmed, Asia, Shafi, Seema, Janes, Sam M., Adams, Haydn, Langman, Gerald, Hafez, Dina, McGranahan, Nicholas, Hackshaw, Allan, Le Quesne, John, Chetty, Mahendran, Swanton, Charles, Summers, Yvonne, Ismail, Babikir, Stewart, Aengus, Grönroos, Eva, Rathinam, Sridhar, Rabinowitz, Matthew, Hiley, Crispin, Oukrif, Dahmane, Jamal-Hanjani, Mariam, Kerr, Keith, Rowan, Andrew, Chambers, Tim, Davies, Helen, Szallasi, Zoltan, Khan, Sajid, Matthews, Nik, Zambrana, Francisco, Peggs, Karl S., Johnson, Diana, Quezada, Sergio A., Laycock, Joanne, Hartley, John A., Lock, Sara, Lowe, Helen L., Irvin-Sellers, Melanie, Abbosh, Christopher, Endozo, Raymondo, Fraioli, Francesco, Birkbak, Nicolai J., Kornaszewska, Malgorzata, Lee, Siow Ming, Lawrence, David, Bell, Harriet, Borg, Elaine, Lester, Jason F., Prakash, Vineet, Schwarz, Roland F., Kareht, Stephanie, Moore, David A., Ward, Sophia, Van Loo, Peter, Kolvekar, Shyam, Shah, Rajesh, Shaw, Jacqui A., Aerts, Hugo J. W. L., Marafioti, Teresa, Herrero, Javier, Martinson, Luke, Akarca, Ayse U., Veeriah, Selvaraju, Turajlic, Samra, Iles, Natasha, Riley,

2017

Overview

The early detection of relapse following primary surgery for non-small-cell lung cancer and the characterization of emerging subclones, which seed metastatic sites, might offer new therapeutic approaches for limiting tumour recurrence. The ability to track the evolutionary dynamics of early-stage lung cancer non-invasively in circulating tumour DNA (ctDNA) has not yet been demonstrated. Here we use a tumour-specific phylogenetic approach to profile the ctDNA of the first 100 TRACERx (Tracking Non-Small-Cell Lung Cancer Evolution Through Therapy (Rx)) study participants, including one patient who was also recruited to the PEACE (Posthumous Evaluation of Advanced Cancer Environment) post-mortem study. We identify independent predictors of ctDNA release and analyse the tumour-volume detection limit. Through blinded profiling of postoperative plasma, we observe evidence of adjuvant chemotherapy resistance and identify patients who are very likely to experience recurrence of their lung cancer. Finally, we show that phylogenetic ctDNA profiling tracks the subclonal nature of lung cancer relapse and metastasis, providing a new approach for ctDNA-driven therapeutic studies. Circulating tumour DNA profiling in early-stage non-small-cell lung cancer can be used to track single-nucleotide variants in plasma to predict lung cancer relapse and identify tumour subclones involved in the metastatic process. Tracking tumour evolution Circulating tumour DNA (ctDNA) has proven useful for detecting and monitoring cancer progression from plasma samples. The authors have applied a bespoke multiplex-PCR next-generation sequencing approach to profile ctDNA in the prospective TRACERx lung cancer clinical trial study. The assay tracks clonal and subclonal variants, in pre- and post-surgery samples. In pre-surgery samples ctDNA detection is associated with histological subtype and other pathological variables and correlates with tumour volume. Blinded longitudinal profiling suggests that ctDNA detection also associates with relapse, and provides insight into the evolutionary patterns of tumour cell subclones during progression. These results advance our understanding of how liquid biopsies can be applied clinically to improve monitoring of cancer.

• Publisher: Nature Publishing Group UK,Nature Publishing Group
• Subject: 45/23 / 59/78 / 631/67/1612/1350 / 631/67/2329 / 631/67/322 / 692/53/2421 / 692/53/2423 / Biopsy - methods / Cancer metastasis / Carcinoma, Non-Small-Cell Lung - blood / Carcinoma, Non-Small-Cell Lung - genetics / Carcinoma, Non-Small-Cell Lung - pathology / Carcinoma, Non-Small-Cell Lung - surgery / Cell Lineage - genetics / Cell Tracking / Clone Cells - metabolism / Clone Cells - pathology / Development and progression / Disease Progression / DNA Mutational Analysis / DNA sequencing / DNA, Neoplasm - blood / DNA, Neoplasm - genetics / Drug Resistance, Neoplasm - genetics / Early Detection of Cancer - methods / Evolution, Molecular / Genetic aspects / Humanities and Social Sciences / Humans / Limit of Detection / Lung cancer / Lung Neoplasms - blood / Lung Neoplasms - genetics / Lung Neoplasms - pathology / Lung Neoplasms - surgery / Methods / multidisciplinary / Multiplex Polymerase Chain Reaction / Neoplasm Metastasis - diagnosis / Neoplasm Metastasis - genetics / Neoplasm Metastasis - pathology / Neoplasm Recurrence, Local - diagnosis / Neoplasm Recurrence, Local - genetics / Neoplasm Recurrence, Local - pathology / Observations / Phylogeny / Postoperative Care - methods / Reproducibility of Results / Science / Tumor Burden