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The distal C terminus of the dihydropyridine receptor β1a subunit is essential for tetrad formation in skeletal muscle
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The distal C terminus of the dihydropyridine receptor β1a subunit is essential for tetrad formation in skeletal muscle
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The distal C terminus of the dihydropyridine receptor β1a subunit is essential for tetrad formation in skeletal muscle
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Journal Article
The distal C terminus of the dihydropyridine receptor β1a subunit is essential for tetrad formation in skeletal muscle
2022
Overview
The skeletal muscle dihydropyridine receptor (DHPR) β1a subunit is indispensable for full trafficking of DHPRs into triadic junctions (i.e., the close apposition of transverse tubules and sarcoplasmic reticulum [SR]), facilitation of DHPRα1S voltage sensing, and arrangement of DHPRs into tetrads as a consequence of their interaction with ryanodine receptor (RyR1) homotetramers. These three features are obligatory for skeletal muscle excitation–contraction (EC) coupling. Previously, we showed that all four vertebrate β isoforms (β₁–β₄) facilitate α1S triad targeting and, except for β₃, fully enable DHPRα1S voltage sensing [Dayal et al., Proc. Natl. Acad. Sci. U.S.A. 110, 7488–7493 (2013)]. Consequently, β₃ failed to restore EC coupling despite the fact that both β₃ and β1a restore tetrads. Thus, all β-subunits are able to restore triad targeting, but only β1a restores both tetrads and proper DHPR–RyR1 coupling [Dayal et al., Proc. Natl. Acad. Sci. U.S.A. 110, 7488–7493 (2013)]. To investigate the molecular region(s) of β1a responsible for the tetradic arrangement of DHPRs and thus DHPR–RyR1 coupling, we expressed loss- and gain-of-function chimeras between β1a and β₄, with systematically swapped domains in zebrafish strain relaxed (β₁-null) for patch clamp, cytoplasmic Ca2+ transients, motility, and freeze-fracture electron microscopy. β1a/β₄ chimeras with either N terminus, SH3, HOOK, or GK domain derived from β₄ showed complete restoration of SR Ca2+ release. However, chimera β1a/β₄(C) with β₄ C terminus produced significantly reduced cytoplasmic Ca2+ transients. Conversely, gain-of-function chimera β₄/β1a(C) with β1a C terminus completely restored cytoplasmic Ca2+ transients, DHPR tetrads, and motility. Furthermore, we found that the nonconserved, distal C terminus of β1a plays a pivotal role in reconstitution of DHPR tetrads and thus allosteric DHPR–RyR1 interaction, essential for skeletal muscle EC coupling.
