Journal Article

Three Unrelated Viral Transforming Proteins (vIRF, EBNA2, and E1A) Induce the MYC Oncogene through the Interferon-Responsive PRF Element by Using Different Transcription Coadaptors

Patrick S. Moore, Ai-En Thlick, Yuan Chang, Sukhanya Jayachandra, Paul D. Ling, Jin Yu, Kenneth G. Low

1999

Overview

Kaposi sarcoma-associated herpesvirus vIRF is a viral transcription factor that inhibits interferon signaling and transforms NIH 3T3 cells, but does not bind interferon-stimulated response element (ISRE) DNA sequences. Here we show that induction of the MYC protooncogene is required for cell transformation by vIRF, and that vIRF increases MYC transcription up to 15-fold through specific promoter interactions at an ISRE sequence called the plasmacytoma repressor factor (PRF) element. These effects are resistant to cycloheximide but are inhibited by a dominant-negative ISRE-binding protein, indicating that vIRF acts together with a cellular cofactor at the PRF element to directly transactivate MYC. The coadaptor CREB-binding protein (CBP) binds vIRF and synergizes transactivation of MYC, but, unexpectedly, closely related histone acetyltransferases p300 and P/CAF potently suppress vIRF transactivation. On the basis of the prediction that other interferon-inhibiting viral transforming proteins behave similarly, we found that Epstein-Barr virus-induced nuclear antigen 2 (EBNA2) also binds p300/CBP, and that both EBNA2 and adenovirus E1A transactivate MYC through the PRF element. For E1A, P/CAF coactivates MYC, whereas both p300 and CBP suppress E1A transactivation. For EBNA2, both P/CAF and CBP coactivate the MYC promoter, whereas p300 suppresses EBNA2 transactivation. These findings demonstrate that viral transforming proteins can activate as well as inhibit transcription through coadaptor interactions. At some promoters CBP and p300 have previously unrecognized, competitive antagonism to each other. While all three viral proteins target the same promoter element, each has a different coadaptor use profile. These findings are consistent with cellular MYC repression playing a role in innate immunity as well as in control of cell proliferation.

• Publisher: National Academy of Sciences of the United States of America,National Acad Sciences,The National Academy of Sciences
• Subject: 3T3 Cells / Adenovirus / Adenovirus E1A Proteins - physiology / Animals / Bacterial Proteins - physiology / Biological Sciences / Cell cycle / Cell lines / Cell Transformation, Neoplastic / CREB-binding protein / Cyclic AMP Response Element-Binding Protein - physiology / cycloheximide / DNA / EBNA2 protein / Epstein-Barr virus / Epstein-Barr Virus Nuclear Antigens - physiology / Genes, myc / Interferons / Interferons - pharmacology / Mice / MYC gene / NIH 3T3 cells / P/CAF protein / p300 protein / plasmacytoma repressor factor / Plasmids / Promoter Regions, Genetic / Proto-Oncogene Proteins c-myc - biosynthesis / Repression / Response Elements / Transactivation / Transcription factors / Transcription, Genetic / Transcriptional Activation / vIRF protein / Virulence Factors