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Aequorea ’s secrets revealed: New fluorescent proteins with unique properties for bioimaging and biosensing
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Aequorea ’s secrets revealed: New fluorescent proteins with unique properties for bioimaging and biosensing
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Journal Article
Aequorea ’s secrets revealed: New fluorescent proteins with unique properties for bioimaging and biosensing
2020
Overview
In addition to transcripts encoding an FP clearly homologous to A. victoria green fluorescent protein (avGFP), as we expected, the A. cf. australis transcriptome also contains several transcripts encoding other, much more divergent avGFP homologs. Photophysical properties of fluorescent proteins described in this study derived from A. victoria and A. cf. australis. https://doi.org/10.1371/journal.pbio.3000936.t001 Intrigued by the diversity of optical properties in the A. cf. australis FP homologs, we next investigated a sample of A. victoria from the Crystal Jelly exhibit at the Birch Aquarium at Scripps to determine whether this species also contained multiple diverse FPs. The data underlying this figure (nucleotide sequences of the FPs from this study) may be found in GenBank, accession numbers MN114103 through MN114112. avGFP, Aequorea victoria green fluorescent protein; FP, fluorescent protein. https://doi.org/10.1371/journal.pbio.3000936.g003 Multiple, diverse Aequorea GFPs As expected, both Aequorea species abundantly express close homologs of avGFP. Though brightly fluorescent, AausFP1 is largely insoluble in this context, and when purified, the soluble fraction of the protein runs as a high-molecular-weight aggregate on size exclusion chromatography (Fig BB in S1 Text).
Publisher
Public Library of Science
Subject
