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The effect of combined herbal bioactive compounds and organic minerals (Fe & Se) on chemical egg quality and production performance of laying hens
The effect of combined herbal bioactive compounds and organic minerals (Fe & Se) on chemical egg quality and production performance of laying hens
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The effect of combined herbal bioactive compounds and organic minerals (Fe & Se) on chemical egg quality and production performance of laying hens
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The effect of combined herbal bioactive compounds and organic minerals (Fe & Se) on chemical egg quality and production performance of laying hens
The effect of combined herbal bioactive compounds and organic minerals (Fe & Se) on chemical egg quality and production performance of laying hens
Journal Article

The effect of combined herbal bioactive compounds and organic minerals (Fe & Se) on chemical egg quality and production performance of laying hens

2026
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Overview
This study evaluated the effects of combining herbal bioactive compounds with organic minerals (Fe and Se) on egg quality, intestinal morphology, and production performance of laying hens. Six dietary treatments (T1–T6), each replicated six times, were arranged in a completely randomized design. Data were analyzed using one-way ANOVA, followed by orthogonal contrast and Bonferroni post hoc tests. The results showed significant effects of treatments on body weight (F = 19.400; p < 0.001), egg mass (F = 3.320; p = 0.020), feed intake (F = 4.205; p = 0.007), and feed conversion ratio (F = 4.141; p = 0.007), while egg weight differences were not significant (p = 0.927). The combination of herbal bioactives with organic Fe and Se minerals enhanced feed efficiency, nutrient absorption, and metabolic performance, as indicated by improved body weight gain and egg mass. Chemically, supplemented hens produced eggs with higher protein levels (albumen 12.03%, yolk 17.40%) and stable organic matter content (∼99%), while maintaining normal yolk cholesterol (702.8 mg/100 g). Mineral biofortification was evident through increased yolk Fe (1.82 mg/100 g) and Se (22.08 µg/100 g) concentrations, accompanied by elevated IgY antibody levels (3163 µg/g), suggesting immunological enhancement. Morphometric observations showed stable liver, gizzard, and heart proportions (CV <10%), indicating consistent metabolic health, while intestinal length uniformity reflected efficient nutrient absorption. Treatment T6 demonstrated the best overall production score (2095.33 ± 47.42), significantly higher than the control (T1 = 1820.67 ± 89.52) and several other treatments (p < 0.05), while T5 exhibited comparable performance (p = 0.343). In conclusion, supplementation with herbal bioactive compounds combined with organic Fe and Se minerals significantly improved egg chemical stability, nutrient composition, feed utilization, and production efficiency. This synergistic formulation represents a sustainable, antibiotic-free strategy for enhancing egg quality and supporting functional, nutrient-enriched egg production in laying hens.