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The Antioxidant Effects of Pumpkin Seeds, Pumpkin Seed Oil and Zinc Against Food Coloring Induced Oxidative Stress and Hepatotoxicity in Rats
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The Antioxidant Effects of Pumpkin Seeds, Pumpkin Seed Oil and Zinc Against Food Coloring Induced Oxidative Stress and Hepatotoxicity in Rats
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Conference Proceeding
The Antioxidant Effects of Pumpkin Seeds, Pumpkin Seed Oil and Zinc Against Food Coloring Induced Oxidative Stress and Hepatotoxicity in Rats
2019
Overview
the present study investigated chemical composition of pumpkin seeds, oil and the protective effect of zinc, pumpkin seeds and pumpkin seeds oil against food coloring induced oxidative stress and hepatotoxicity in rats for three months. Thirty six male albino rats (Sprague Dawley strain) weight 140± 10g gm were investigated. Rats were divided into six groups (six rats of each). The first group (6 rats) was considerable as negative control and it was fed on basal diet .The second group fed on basal diet supplemented with (75 mg/kg diet from tartrazine& 0.4 g/ kg diet from brilliant blue (as a positive control group).The third group fed on basal diet supplemented with food colors + pumpkin seeds (25g/ kg diet). The fourth group fed on basal diet basal diet supplemented with food colors + pumpkin seeds oil (8mg/ kg diet). The fifth group fed on basal diet supplemented with food colors + pumpkin seeds (25g/ kg diet) + (25 mg Zn/g diet).The sixth group fed on basal diet supplemented with food colors + pumpkin seeds oil (8mg/kg diet) + (25 mg Zn/g diet). At the end of the experiment biological data were calculated, blood samples were taken. Serum was separated to biochemical analysis. The results revealed that the chemical composition of pumpkin seeds indicates the presence of high amounts of lipids, protein, crude fiber, total carbohydrates, ash, zinc and also low moisture content, respectively. The control (+ve) group showed a significant decrease in weight gain; liver function, kidney function, liver GPX & SOD and catalase, but a significant increase in liver LPX compared with control (- ve) group. Serum total cholesterol and LDL-C showed significant increase of C+ve group, but all treated groups with PS, PSO, PS+Z and PSO+Z showed improvement in liver function , kidney function ,lipid profile, liver GPX& SOD and catalase compared with C+ve group. In conclusion, the administration of pumpkin seeds, pumpkin seeds oil and zinc can lower the side effects of food colors toxicant, have antioxidant and hepatoprotective potential.
Publisher
جامعة المنصورة - كلية التربية النوعية
Subject
