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De novo assembly and sex-specific transcriptome profiling in the sand fly Phlebotomus perniciosus (Diptera, Phlebotominae), a major Old World vector of Leishmania infantum
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De novo assembly and sex-specific transcriptome profiling in the sand fly Phlebotomus perniciosus (Diptera, Phlebotominae), a major Old World vector of Leishmania infantum
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De novo assembly and sex-specific transcriptome profiling in the sand fly Phlebotomus perniciosus (Diptera, Phlebotominae), a major Old World vector of Leishmania infantum
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Journal Article
De novo assembly and sex-specific transcriptome profiling in the sand fly Phlebotomus perniciosus (Diptera, Phlebotominae), a major Old World vector of Leishmania infantum
2015
Overview
Background
The phlebotomine sand fly
Phlebotomus perniciosus
(Diptera: Psychodidae, Phlebotominae) is a major Old World vector of the protozoan
Leishmania infantum
, the etiological agent of visceral and cutaneous leishmaniases in humans and dogs, a worldwide re-emerging diseases of great public health concern, affecting 101 countries. Despite the growing interest in the study of this sand fly species in the last years, the development of genomic resources has been limited so far. To increase the available sequence data for
P. perniciosus
and to start studying the molecular basis of the sexual differentiation in sand flies, we performed whole transcriptome Illumina RNA sequencing (RNA-seq) of adult males and females and
de novo
transcriptome assembly.
Results
We assembled 55,393 high quality transcripts, of which 29,292 were unique, starting from adult whole body male and female pools. 11,736 transcripts had at least one functional annotation, including full-length low abundance salivary transcripts, 981 transcripts were classified as putative long non-coding RNAs and 244 transcripts encoded for putative novel proteins specific of the Phlebotominae sub-family. Differential expression analysis identified 8590 transcripts significantly biased between sexes. Among them, some show relaxation of selective constraints when compared to their orthologs of the New World sand fly species
Lutzomyia longipalpi
s.
Conclusions
In this paper, we present a comprehensive transcriptome resource for the sand fly species
P. perniciosus
built from short-read RNA-seq and we provide insights into sex-specific gene expression at adult stage. Our analysis represents a first step towards the identification of sex-specific genes and pathways and a foundation for forthcoming investigations into this important vector species, including the study of the evolution of sex-biased genes and of the sexual differentiation in phlebotomine sand flies.
Publisher
BioMed Central,BioMed Central Ltd,Springer Nature B.V
Subject
/ Animal Genetics and Genomics
/ Animals
/ Biomedical and Life Sciences
/ Dogs
/ Female
/ Genes
/ High-Throughput Nucleotide Sequencing
/ Humans
/ Leishmania infantum - genetics
/ Leishmania infantum - pathogenicity
/ Leishmaniasis, Visceral - genetics
/ Leishmaniasis, Visceral - parasitology
/ Male
/ Methods
/ Microbial Genetics and Genomics
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