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Colorful Packages: Fluorescent Proteins in Complex Coacervate Core Micelles
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Colorful Packages: Fluorescent Proteins in Complex Coacervate Core Micelles
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Dissertation
Colorful Packages: Fluorescent Proteins in Complex Coacervate Core Micelles
2018
Overview
During this PhD-project the composition, formation, dynamics, and stability of complex coacervate core micelles (C3Ms) containing fluorescent proteins have been explored. The project started with encapsulation of enhanced green fluorescent protein (EGFP) in C3Ms and the characterization of these micelles using dynamic light scattering (DLS) and fluorescence correlation spectroscopy (FCS). The potential of these two techniques for the C3M research is analyzed in Chapter 2. Subsequently, the consequences of encapsulation on the structure and spectral properties of EGFP were investigated (Chapter 3). In Chapter 4 fluorescent proteins (FPs) from different classes (Hydrozoa and Anthozoa), covering the whole visible spectrum, were encapsulated in C3Ms. In Chapter 5, C3M dynamics were investigated by using two selected FPs. Finally, in Chapter 6, the results of this project are placed into perspective with respect to their implications for the application of protein-containing C3Ms in biotechnology and medicine. Furthermore, directions for future research are discussed.
