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Fluorescence Quenching Analysis of 6MNPM Molecule by Steady State Method
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Fluorescence Quenching Analysis of 6MNPM Molecule by Steady State Method
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Fluorescence Quenching Analysis of 6MNPM Molecule by Steady State Method
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Journal Article
Fluorescence Quenching Analysis of 6MNPM Molecule by Steady State Method
2025
Overview
The current work uses aniline as a quencher in a variety of solvents to investigate the room-temperature fluorescence quenching of a physiologically active fluorescent probe, namely '6-Methoxy-4-(4-nitro-phenoxy methyl)-chromene-2-one (6MNPM) coumarin molecule'. To understand its behaviour in various media, several solvents with different dielectric constants and refractive indices have been used. Spectroscopy techniques and time-correlated single photon counting were used to describe the absorption spectra, emission spectra, and lifespan value of a molecule. From fluorescence quenching analysis, we find that the S-V curve shows a linear dependence in given solvents with various dielectric values. It is shown that quenching responses are dynamic, many forms of quenching have been identified, and the relevant parameters have been assessed.
Publisher
CHRIST (Deemed to be University)
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