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In the context of the ever-expanding global reach of Chinese film and television productions, ancient costume dramas, as a cinematic and televisual genre infused with prominent Chinese cultural traits, have come under intense scrutiny. The Story of Minglan , in particular, stands out as a prime exemplar within this genre. This research focuses on the subtitle translation of this drama and deeply analyzes its translation strategies and practical effects from a cross-cultural perspective. On one hand, this research analyzes the translation of numerous culture-loaded terms in the drama, which are rich and unique and cover aspects. On the other hand, this study delves into the translation methods for various forms of language art. By analyzing typical subtitle translation cases in the drama (mainly based on YouTube translations), this study reveals the crucial role of cross-cultural subtitle translation in promoting cultural exchange and breaking down cultural barriers. It provides translation ideas and methods of reference for Chinese ancient costume dramas and even more film and television works rich in profound cultural connotations to go global, thus facilitating the international dissemination of outstanding traditional Chinese culture.

Our previous work demonstrated that the anisodamine (ANI) and neostigmine (NEO) combination produced an antiseptic shock effect and rescued acute lethal crush syndrome by activating the α7 nicotinic acetylcholine receptor (α7nAChR). This study documents the therapeutic effect and underlying mechanisms of the ANI/NEO combination in dextran sulfate sodium (DSS)-induced colitis. Treating mice with ANI and NEO at a ratio of 500:1 alleviated the DSS-induced colitis symptoms, reduced body weight loss, improved the disease activity index, enhanced colon length, and alleviated colon inflammation. The combination treatment also enhanced autophagy in the colon of mice with DSS-induced colitis and lipopolysaccharide/DSS-stimulated Caco-2 cells. Besides, the ANI/NEO treatment significantly reduced INF-γ, TNF-α, IL-6, and IL-22 expression in colon tissues and decreased TNF-α, IL-1β, and IL-6 mRNA levels in Caco-2 cells. Meanwhile, the autophagy inhibitor 3-methyladenine and ATG5 siRNA attenuated these effects. Furthermore, 3-methyladenine (3-MA) and the α7nAChR antagonist methyllycaconitine (MLA) weakened the ANI/NEO-induced protection on DSS-induced colitis in mice. Overall, these results indicate that the ANI/NEO combination exerts therapeutic effects through autophagy and α7nAChR in a DSS-induced colitis mouse model.

BackgroundSystemic sclerosis (SSc) is an autoimmune disease. In the types of SSc, lcSSc presents with milder symptoms and poses challenges for early diagnosis. Therefore, the identification of biomarkers for earlier diagnosis and the discovery of potential therapeutic targets are of greater significance. This study aims to explore the relationship between competing endogenous RNA (ceRNA) networks, cuproptosis, and lcSSc.MethodsRNA sequencing was performed to profile differentially expressed RNAs in blood samples from lcSSc patients and healthy controls. The potential ceRNA network targeting relationships were then examined using a dual-luciferase reporter assay. Subsequently, a series of in vitro experiments utilizing primary CD8+ T cells. These experiments, which included phenotypic assays and molecular analyses, demonstrated the occurrence of cuproptosis. Finally, these findings were validated in vivo in a mouse model through analyses of skin tissues using H&E staining, Masson staining, Immunofluorescence, qPCR, and Western blotting.ResultsTTLL7-IT1/hsa-miR-29c-3p/GLS was constructed using bioinformatic analysis of lcSSc. CD8+ T cells from patients with lcSSc exhibited elevated copper ion levels and reduced glutaminase(GLS) expression, accompanied by alterations in their phenotype and cuproptosis-related proteins. These phenotypic and protein expression changes were reversed by cuproptosis inhibition. Furthermore, cuproptosis exacerbates fibrosis and vascular pathology. Animal experiments further revealed that in bleomycin-induced mouse models, both inhibition of copper ions and GLS overexpression reduced dermal thickness, collagen deposition, fibrotic factor expression, and CD31/α-SMA co-localization in the skin.ConclusionsIn summary, our findings reveal a functional interaction between cuproptosis and ceRNA networks: TTLL7-IT1/hsa-miR-29c-3p/GLS influences cuproptosis and lcSSc progression, providing new insights into the pathogenesis of lcSSc.

The rapid repurposing of antivirals is particularly pressing during pandemics. However, rapid assays for assessing candidate drugs typically involve in vitro screens and cell lines that do not recapitulate human physiology at the tissue and organ levels. Here we show that a microfluidic bronchial-airway-on-a-chip lined by highly differentiated human bronchial-airway epithelium and pulmonary endothelium can model viral infection, strain-dependent virulence, cytokine production and the recruitment of circulating immune cells. In airway chips infected with influenza A, the co-administration of nafamostat with oseltamivir doubled the treatment-time window for oseltamivir. In chips infected with pseudotyped severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), clinically relevant doses of the antimalarial drug amodiaquine inhibited infection but clinical doses of hydroxychloroquine and other antiviral drugs that inhibit the entry of pseudotyped SARS-CoV-2 in cell lines under static conditions did not. We also show that amodiaquine showed substantial prophylactic and therapeutic activities in hamsters challenged with native SARS-CoV-2. The human airway-on-a-chip may accelerate the identification of therapeutics and prophylactics with repurposing potential. A microfluidic bronchial-airway-on-a-chip lined by human bronchial-airway epithelium and pulmonary endothelium can be used to rapidly identify antiviral therapeutics and prophylactics with repurposing potential.

Diamond wire sawing is widely applied to hard and brittle materials due to its narrow kerf and flexibility. Introducing longitudinal ultrasonic vibration further improves machining performance and surface quality. A longitudinal ultrasonic vibration–assisted diamond wire sawing system was developed using an ultrasonic guide wheel to efficiently transmit vibration to the wire, and its vibration behavior was verified by laser vibrometry. Cutting experiments on ZrO 2 ceramics compared ultrasonic-assisted and conventional processes. The results demonstrate that, under appropriate ultrasonic power and wire speed, ultrasonic assistance significantly reduces surface roughness and scratch depth while suppressing chip adhesion, improving overall surface quality.

Diamond wire sawing is widely applied to hard and brittle materials due to its narrow kerf and flexibility. Introducing longitudinal ultrasonic vibration further improves machining performance and surface quality. A longitudinal ultrasonic vibration–assisted diamond wire sawing system was developed using an ultrasonic guide wheel to efficiently transmit vibration to the wire, and its vibration behavior was verified by laser vibrometry. Cutting experiments on ZrO2 ceramics compared ultrasonic-assisted and conventional processes. The results demonstrate that, under appropriate ultrasonic power and wire speed, ultrasonic assistance significantly reduces surface roughness and scratch depth while suppressing chip adhesion, improving overall surface quality.

PurposeThis study aimed to investigate the safety and efficacy of three-dimensional (3D) surgical video systems for proliferative diabetic retinopathy (PDR).MethodsThis retrospective clinical case study included 30 patients (30 eyes) with PDR. Patients were divided into two groups: one underwent surgery using a 3D surgical video system (14 cases, 14 eyes), while the other underwent traditional microscope surgery (16 cases, 16 eyes). Safety and efficacy were assessed through predetermined surgical parameters, including surgical duration, intraoperative membrane removal rate, and occurrences during intraoperative and postoperative phases.ResultsOur study revealed noteworthy differences in various aspects between the 3D surgical video system group and the traditional microscope surgery group. Specifically, the mean surgical time was 30.25 ± 14.43 mins in the 3D surgical video system group, while it was 38.56 ± 18.71 mins in the traditional microscope surgery group ( p = 0.051). Furthermore, the mean membrane removal time was significantly shorter in the 3D group at 2.53 ± 1.52 mins, as compared to 3.23 ± 1.76 mins in the traditional group ( p = 0.042). Importantly, the membrane removal rate also displayed a significant difference, with the 3D group at 0.55 ± 0.07 and the traditional group at 0.41 ± 0.11 ( p = 0.018). However, no notable differences were observed between the two groups in terms of intraoperative and postoperative incidences.ConclusionThe safety and efficacy obtained using the 3D surgical video system in PDR surgery were comparable to those obtained in traditional microscopic surgery.

Recurrence of breast cancer after radiotherapy may be partly explained by the presence of radioresistant cells. Thus, it would be desirable to develop an effective therapy against radioresistant cells. In this study, we demonstrated the intense antitumor activity of cytokine-induced killer cells against MCF-7 and radioresistant MCF-7 cells, as revealed by cytokine-induced killer–mediated cytotoxicity, tumor cell proliferation, and tumor invasion. Radioresistant MCF-7 cells were more susceptible to cytokine-induced killer cell killing. The stronger cytotoxicity of cytokine-induced killer cells against radioresistant MCF-7 cells was dependent on the expression of major histocompatibility complex class I polypeptide–related sequence A/B on radioresistant MCF-7 cells after exposure of cytokine-induced killer cells to sensitized targets. In addition, we demonstrated that cytokine-induced killer cell treatment sensitized breast cancer cells to chemotherapy via the downregulation of TK1, TYMS, and MDR1. These results indicate that cytokine-induced killer cell treatment in combination with radiotherapy and/or chemotherapy may induce synergistic antitumor activities and represent a novel strategy for breast cancer.

Depression as a mental illness is commonly observed to co-occur with various somatic diseases, such as gastrointestinal diseases. However, previous studies have primarily focused on the risk of mental disorders following physical illnesses. Our study took depression as a risk factor, attempting to explore its relationship with gastrointestinal diseases. A total of 457,940 participants (aged 37-73 years) in the UK Biobank were included. The Cox proportional hazards model was used to assess the relationship between depression and gastrointestinal diseases. Mendelian randomization assessed the causal link between depression and gastrointestinal disorders, and seven machine learning algorithms (including LightGBM, XGBoost, and Random Forest) were trained in the total population to develop predictive models for incident gastrointestinal diseases, with model performance evaluated using the area under the receiver operating characteristic curve (AUC). During a median follow-up period of 13.7 years, 9563 esophagitis events, 36,420 gastroesophageal reflux disease events, 5469 gastric ulcer events, 3096 duodenal ulcer events, 37,225 gastritis and duodenitis events, and 9153 dyspepsia events were recorded. After adjusting for covariates, depression was associated with increased risk of all six diseases. Two-sample MR analysis supported a causal association. Machine learning models demonstrated good discrimination, with the highest predictive accuracy observed for duodenal ulcer (AUC = 0.76) and gastric ulcer (AUC = 0.75). Addressing depression as a modifiable risk factor may reduce gastrointestinal disease risk, especially in disadvantaged populations, by integrating mental health care into primary care and using predictive models for early intervention.

Previous studies have investigated the association between diet and cognitive impairment, yet there is limited investigation into the link between edible mushrooms and algae intake and cognitive decline. This study aims to explore the association between edible mushrooms and algae intake and the risk of cognitive impairment in individuals aged 65 years and above in China. Cross-sectional data from the 2018 Chinese Longitudinal Healthy Longevity Survey (CLHLS) formed the basis of this study. Edible mushrooms and algae intake was evaluated using a simplified food frequency questionnaire (FFQ) and cognitive function was assessed using the Mini-Mental State Examination (MMSE). A binary logistic regression model was used to evaluate odds ratios (ORs) and 95% confidence intervals (CIs), with subgroup analysis conducted. Among 14,150 older adults, the average age was (85.33 ± 11.55), with a cognitive impairment prevalence of 22.7; multi-model adjustments showed a 25.3% lower probability of cognitive impairment for those occasionally consuming edible mushrooms and algae (OR: 0.747, 95% CI: 0.675~0.826). Furthermore, a 29% lower risk was observed in those with daily intake (OR: 0.710, 95% CI: 0.511~0.987). Subgroup analysis demonstrated significant risk reduction in women (OR: 0.589, 95% CI: 0.375~0.925, p = 0.022), individuals with disability in activities of daily living (OR: 0.568, 95% CI: 0.367~0.878, p = 0.011), and those with low social activity levels (OR: 0.671, 95% CI: 0.473~0.950, p = 0.025). This study concludes that edible mushrooms and algae intake significantly impacts the risk of cognitive impairment in older adults. These results provide insights and impetus for further research into this area. Additional cohort studies or intervention trials are necessary to confirm the potential benefits of edible mushrooms and algae in promoting cognitive health.