Journal Article

HIV-1 Vpu interacts with RBM10 to promote HIV-1 infection

Yang, Yishu, Yu, Boyang, Jia, Runqing, Hao, Yanzhe, Zhang, Xiaoguang, Chen, Tian, Meng, Xianbin, Wang, Xiayan, Li, Boye, Li, Qingyan, Hu, Qin, Yang, Jinning, Wang, Xiaoming, Zhang, Wenmei

2025

Overview

A comprehensive analysis utilizing APEX2-MS and IP-MS techniques identified a total of 24 cellular targets of Vpu, three of which have been documented as restriction factors. Vpu-interacting proteins were found to be significantly enriched in pathways related to cell adhesion, RNA transport, and the spliceosome. The identification of RBM10 as a novel regulator of HIV-1 replication and infectivity, and RBM10 regulated transcription of both viral and host RNA transcripts. Vpu interacted with RBM10 and promoted its degradation through the ubiquitin-proteasome pathway.

• Publisher: American Society for Microbiology
• Subject: Acquired immune deficiency syndrome / AIDS / Antigens / Ascorbic acid / Cell adhesion / Cell adhesion & migration / Cell culture / Dengue fever / Endoplasmic reticulum / engineered ascorbate peroxidase labeling / HEK293 Cells / HIV / HIV Infections - metabolism / HIV Infections - virology / HIV-1 - genetics / HIV-1 - metabolism / HIV-1 - physiology / Host-Microbial Interactions / Host-Pathogen Interactions / Human immunodeficiency virus / Human Immunodeficiency Virus Proteins - metabolism / Humans / Immune system / Immunoprecipitation / Immunosurveillance / Infections / Infectivity / L-Ascorbate peroxidase / Labeling / Lymphocytes / Mass spectroscopy / Pathogenesis / Penicillin / Plasmids / Proteasomes / Protein Binding / protein-protein interaction / Proteins / Reagents / Replication / Research Article / RNA transport / RNA, Viral - metabolism / RNA-binding motif protein 10 / RNA-Binding Proteins - genetics / RNA-Binding Proteins - metabolism / Transcription / Ubiquitin / viral protein U / Viral Regulatory and Accessory Proteins - metabolism / Viroporin Proteins / Virus Replication / Viruses