Journal Article

Multiple herbicide–resistant Lolium spp. is prevalent in wheat production in Texas Blacklands

Abugho, Seth, Swart, James, Singh, Vijay, Drake, David, Bagavathiannan, Muthukumar, Maity, Aniruddha

2020

Overview

Field surveys were conducted across the Blacklands region of Texas during 2016 and 2017 to document the distribution of herbicide-resistant Lolium spp. infesting winter wheat production fields in the region. A total of 68 populations (64 Italian ryegrass, four perennial ryegrass) were evaluated in a greenhouse for sensitivity to herbicides of three different modes of action: an acetolactate synthase (ALS) inhibitor (mesosulfuron-methyl), two acetyl-coenzyme-A carboxylase (ACCase) inhibitors (diclofop-methyl and pinoxaden), and a 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) inhibitor (glyphosate). Herbicides were applied at twice the label-recommended rates for mesosulfuron-methyl (29 g ai ha–1), diclofop-methyl (750 g ai ha–1), and pinoxaden (118 g ai ha–1); and at the recommended rate for glyphosate (868 g ae ha–1). The herbicide screenings were followed by dose-response assays of the most-resistant ryegrass population for each herbicide at eight rates (0.5, 1, 2, 4, 8, 16, 32, and 64×), compared with a susceptible population at six rates (0.0625, 0.125, 0.25, 0.5, 1, and 2×). The initial screening and dose-response experiments were conducted in a completely randomized design with three replications and two experimental runs. Survivors (<80% injury) were characterized as highly resistant (0% to 20% injury) or moderately resistant (21% to 79%). Results showed that 97%, 92%, 39%, and 3% of the Italian ryegrass populations had survivors to diclofop-methyl, mesosulfuron-methyl, pinoxaden, and glyphosate treatments, respectively. Of the four perennial ryegrass populations, three were resistant to diclofop-methyl and mesosulfuron-methyl, and one was resistant to pinoxaden as well. Perennial ryegrass populations did not exhibit any resistance to glyphosate. Dose-response assays revealed 37-, 196-, and 23-fold resistance in Italian ryegrass to mesosulfuron-methyl, diclofop-methyl, and pinoxaden, respectively, compared with a susceptible standard. One Italian ryegrass population exhibited three-way multiple resistance to ACCase-, ALS-, and EPSPS-inhibitors. The proliferation of multiple herbicide–resistant ryegrass is a challenge to sustainable wheat production in Texas Blacklands and warrants diversified management strategies.

• Publisher: Cambridge University Press
• Subject: 3-phosphoshikimate 1-carboxyvinyltransferase / Acetolactate synthase / acetyl coenzyme A / administrative management / assays / at-risk population / Crop production / Crop science / design / diclofop-methyl / Dose-response / Enzymes / Fatty acids / fields / Glyphosate / glyphosate resistance / greenhouses / Herbicides / Inhibitors / Lolium / Lolium multiflorum / Lolium perenne / mechanism of action / multiple herbicide resistance / pesticide application / Plant growth / Population / Populations / resistance spread / screening / semistratified sampling / surveys / Sustainable production / technology / Texas / weed resistance survey / Wheat / Winter wheat