Asset Details
Do you wish to reserve the book?
Which Nests to Choose: Collecting Shed Hairs from Wild Orang-Utans
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Which Nests to Choose: Collecting Shed Hairs from Wild Orang-Utans
Do you wish to request the book?
Which Nests to Choose: Collecting Shed Hairs from Wild Orang-Utans
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Which Nests to Choose: Collecting Shed Hairs from Wild Orang-Utans
2004
Overview
The use of non-invasive genetics to study great apes has become increasingly common [Gerloff et al., 1999; Vigilant et al., 2001; Utami et al., 2002; Clifford et al., 2003]. Apes are known to build a new nest every night and hair shed during the night can be easily found in these platforms but, where nests are built high in trees, shed-hair collection can be both difficult and dangerous. Moreover, DNA extracted from shed hairs can be degraded and in low quantities [Gagneux et al., 1997]. Low amounts of DNA can generate unreliable data such as allelic dropout and false alleles during the amplification of genetic markers such as microsatellites [Gagneux et al., 1997; Taberlet et al., 1999]. Despite the fact that new techniques [Morin et al., 2001] and simulation software [Valiere et al., 2002] have been published and can be used for accurate microsatellite genotyping from non-invasive samples, the quality of the samples must not be neglected. DNA is present only in the roots of shed hairs, and previous studies have shown that an increase in the number of roots in a hair sample increases microsatellite genotyping reliability [e.g. Gagneux et al., 1997]. In this paper, we present evidence that hairs shed by orang-utans should ideally be collected from fresh nests between 1 and 5 nights old. We also suggest that 1-night-old nests are preferable, due to the opportunity to find fresh faeces below them.
Publisher
Brill,Karger,S. Karger AG
Subject
