Journal Article

MOLECULAR CHARACTERIZATION OF ISOSPOROID COCCIDIA (ISOSPORA AND ATOXOPLASMA SPP.) IN PASSERINE BIRDS

Tokarz, Debra, Keener, Laura L., Gaffney, Patricia M., Maalouf, Gabriel A., McAloose, D., Schrenzel, Mark D., Griffey, Stephen, McClure, Diane, Rideout, Bruce A.

2005

Overview

Prevalence and disease caused by isosporoid coccidia in passerine birds are well recognized, but confusion about the life cycles of the parasites has led to taxonomic inconsistencies. In this study, we characterized segments of the chromosomal small and large-subunit ribosomal RNA (rRNA) genes of coccidial parasites from 23 species of passerine birds, as well as heat shock protein 70, apicoplast rRNA, and chromosomal 5.8s rRNA genes from a subgroup of these animals, and we correlated genetic data with morphologic findings for different parasite developmental stages, host phylogeny, and overall taxonomic relations within the phylum Apicomplexa. Our findings indicate that isosporoid coccidia of passerine birds are monophyletic but exhibit substantial diversity, with most avian species having one or several unique parasite lineages that underwent synchronous speciation with their hosts, interrupted by sporadic episodes of lateral transmission across species and families. Molecular analyses support a homoxenous life cycle, with sexual forms occurring chiefly in the intestines and asexual merozoites present systemically. Rarely, extraintestinal sexual stages can occur. The passerine coccidia are genetically most closely related to species of Eimeria rather than Isospora. We suggest that these parasites, whether identified from blood merozoite stages or fecal oocysts, be provisionally grouped as a homogeneous clade of individual species in a single taxon and formally named when reliable criteria allowing reclassification of related genera in the suborder Eimeriina are clarified.

• Publisher: American Society of Parasitologists,Allen Press Inc
• Subject: Animals / Aves / Base Sequence / Biological and medical sciences / Bird Diseases - parasitology / Birds / Coccidia / coccidiosis / Coccidiosis - parasitology / Coccidiosis - veterinary / Developmental stages / Disease transmission / DNA polymerase / DNA, Protozoan - chemistry / DNA, Protozoan - isolation & purification / Eimeriidae - classification / Eimeriidae - genetics / Eimeriidae - ultrastructure / Feces / Fundamental and applied biological sciences. Psychology / Genera / General aspects / General aspects and techniques. Study of several systematic groups. Models / Genes / Genotype / Genotypes / Heat shock proteins / host specificity / HSP-70 gene / HSP70 Heat-Shock Proteins - genetics / Hsp70 protein / Intestine / Invertebrates / Isospora / Isospora - classification / Isospora - genetics / Isospora - ultrastructure / life cycle (organisms) / Life cycles / Merozoites / Molecular Sequence Data / molecular systematics / nucleotide sequences / Oocysts / Parasite hosts / Parasites / Parasitology / Passeriformes / Passeriformes - parasitology / Phylogeny / Polymerase Chain Reaction - veterinary / Reclassification / ribosomal DNA / RNA, Ribosomal - genetics / rRNA / rRNA 5.8S / rRNA genes / Sequence Alignment / Sexual stages / Songbirds / Sparrows / Speciation / Species / Species diversity / Spleen / Subgroups / SYSTEMATICS-PHYLOGENETICS / Taxonomy / ultrastructure / Vertebrates: general zoology, morphology, phylogeny, systematics, cytogenetics, geographical distribution