Journal Article

Periodontitis induced by Porphyromonas gingivalis drives periodontal microbiota dysbiosis and insulin resistance via an impaired adaptive immune response

Kemoun, Philippe, Nicolas, Simon, Blasco-Baque, Vincent, Azalbert, Vincent, Escoula, Quentin, Waget, Aurélie, Pomié, Céline, Le Gall-David, Sandrine, Klopp, Pascale, Bonnaure-Mallet, Martine, Garidou, Lucile, Loubieres, Pascale, Lemaitre, Mathieu, Serino, Matteo, Colom, André, Burcelin, Rémy

2017

Overview

ObjectiveTo identify a causal mechanism responsible for the enhancement of insulin resistance and hyperglycaemia following periodontitis in mice fed a fat-enriched diet.DesignWe set-up a unique animal model of periodontitis in C57Bl/6 female mice by infecting the periodontal tissue with specific and alive pathogens like Porphyromonas gingivalis (Pg), Fusobacterium nucleatum and Prevotella intermedia. The mice were then fed with a diabetogenic/non-obesogenic fat-enriched diet for up to 3 months. Alveolar bone loss, periodontal microbiota dysbiosis and features of glucose metabolism were quantified. Eventually, adoptive transfer of cervical (regional) and systemic immune cells was performed to demonstrate the causal role of the cervical immune system.ResultsPeriodontitis induced a periodontal microbiota dysbiosis without mainly affecting gut microbiota. The disease concomitantly impacted on the regional and systemic immune response impairing glucose metabolism. The transfer of cervical lymph-node cells from infected mice to naive recipients guarded against periodontitis-aggravated metabolic disease. A treatment with inactivated Pg prior to the periodontal infection induced specific antibodies against Pg and protected the mouse from periodontitis-induced dysmetabolism. Finally, a 1-month subcutaneous chronic infusion of low rates of lipopolysaccharides from Pg mimicked the impact of periodontitis on immune and metabolic parameters.ConclusionsWe identified that insulin resistance in the high-fat fed mouse is enhanced by pathogen-induced periodontitis. This is caused by an adaptive immune response specifically directed against pathogens and associated with a periodontal dysbiosis.

• Publisher: BMJ Publishing Group LTD,BMJ Publishing Group
• Subject: Adaptive Immunity / Adoptive transfer / Alveolar bone / Animal models / Animals / Arthritis / Atherosclerosis / Bacteroidaceae Infections - complications / Biofilms / Bone loss / Carbohydrates / Cell Transplantation / Diabetes / Diet / Diet, High-Fat / Disease / Disease Models, Animal / Dysbacteriosis / Dysbiosis - immunology / Dysbiosis - microbiology / Dysbiosis - prevention & control / Female / Fusobacterium nucleatum / Gingiva - microbiology / Glucose metabolism / Gum disease / Gut Microbiota / Homeostasis / Human health and pathology / Hyperglycemia / Hyperglycemia - immunology / Hyperglycemia - microbiology / Hépatology and Gastroenterology / Immune response / Immune system / Inflammation / Insulin / Insulin resistance / Insulin Resistance - immunology / Interferon-gamma - blood / Interleukin-6 - blood / Intestinal microflora / Life Sciences / Lipopolysaccharides / Lipopolysaccharides - immunology / Lymph Nodes - cytology / Lymphocytes / Metabolic disorders / Mice / Mice, Inbred C57BL / Microbiota / Pathogens / Periodontitis / Periodontitis - immunology / Periodontitis - microbiology / Periodontitis - pathology / Periodontitis - prevention & control / Porphyromonas gingivalis / Porphyromonas gingivalis - immunology / Prevotella intermedia / Random Allocation / Spleen - cytology / Testing laboratories / Tumor necrosis factor-TNF / Vaccination