Journal Article

Effect of triamcinolone acetonide on proliferation of retinal endothelial cells in vitro and in vivo

Jonas, J B, Spandau, U H M, Sauder, G, Schubert, U, Hammes, H-P

2005

Overview

Aim: To assess the effect of crystalline triamcinolone acetonide on retinal endothelial cell proliferation in vivo and in vitro. Methods: For in vitro analysis, a sprouting assay was employed. Bovine retinal endothelial cells were stimulated with basic fibroblast growth factor (bFGF) and incubated with different concentrations of triamcinolone acetonide (0.05 mg/ml to 8 mg/ml). For in vivo analysis, a retinopathy of prematurity (ROP) model was used. 16 C57BL/J6 mice were exposed to 75% oxygen from postnatal day 7 to day 12. On day 12, triamcinolone acetonide was intravitreally injected into one eye (“study eye”) and isotonic saline into the contralateral eye (“control eye”). On day 17, the mice were sacrificed and the eyes removed for quantitative analysis of preretinal neovascularisation. Four non-exposed mice served as negative control. Results: The sprouting assay demonstrated a dose dependent inhibition of bovine retinal endothelial cell proliferation from 0.05 mg triamcinolone acetonide/ml (no inhibition) to 3 mg triamcinolone acetonide/ml (complete inhibition). Dosages of more than 2 mg/ml resulted in cytotoxic changes of endothelial cells. The ROP model demonstrated a significantly lower neovascular cell count of 58% in the study group compared to the control group (6.35 (SD 2.1) cells per histological section versus 14.9 (SD 5.3) cells; p<0.005). Conclusions: Triamcinolone acetonide inhibits bFGF induced proliferation of retinal endothelial cells in vivo and in vitro. These findings contribute to understanding the mode of action and effects of triamcinolone acetonide on retinal neovascularisation.

• Publisher: BMJ Publishing Group Ltd,BMJ,BMJ Publishing Group LTD,Copyright 2005 British Journal of Ophthalmology
• Subject: age related macular degeneration / Angiogenesis / Animals / Animals, Newborn / ARMD / basic fibroblast growth factor / bFGF / Biological and medical sciences / bovine retinal endothelial cells / BRECs / Cattle / Cell Proliferation - drug effects / Cells, Cultured / Cytotoxicity / Diabetes / Diabetic retinopathy / Disease Models, Animal / Dose-Response Relationship, Drug / ECGS / endothelial cell growth supplement / Endothelial Cells - cytology / Endothelial Cells - drug effects / Endothelium, Vascular - cytology / Endothelium, Vascular - drug effects / Experiments / Eye / FCS / fetal calf serum / Fibroblast Growth Factor 2 - pharmacology / Fibroblasts / Glucocorticoids - pharmacology / Glucocorticoids - therapeutic use / Humans / Infant, Newborn / Laboratory Science - Scientific Reports / Medical sciences / Mice / Mice, Inbred C57BL / microcarrier / PBS / Pharmacology. Drug treatments / phosphate buffered saline / Retina / retinal endothelial cells / Retinal Neovascularization - etiology / Retinal Neovascularization - pathology / Retinal Neovascularization - prevention & control / Retinal Vessels - cytology / Retinal Vessels - drug effects / retinopathy of prematurity / Retinopathy of Prematurity - complications / Retinopathy of Prematurity - pathology / ROP / Statistical analysis / Studies / triamcinolone acetonide / Triamcinolone Acetonide - pharmacology / Triamcinolone Acetonide - therapeutic use / Vascular endothelial growth factor / VEGF