Journal Article

Isolation and characterization of Methanoculleus receptaculi sp. nov. from Shengli oil field, China

Deng, Yu, Zhang, Hui, Wang, Wei-Dong, Qiu, Tian-Lei, Cheng, Lei, Yin, Xiao-Bo, Li, Xia

2008

Overview

Three strictly anaerobic, thermophilic methanogens (ZC-2T, ZC-3 and ZC-6) were isolated from Shengli oil field, China. The 16S rRNA gene sequences of the three strains were nearly identical, possessing >99.8% sequence similarity. They also possessed high sequence similarity, 97.4%, to Methanoculleus palmolei strain INSLUZT (97.4% and 97.5%, respectively), indicating that they represented a novel species within the genus Methanoculleus. Cells of strain ZC-2T were nonmotile cocci, 0.8-1.7 μm in diameter, and always occurred singly or in pairs. The three strains used H₂/CO₂ or sodium formate as substrates for methanogenesis but not sodium acetate, trimethylamine, monomethylamine, ethanol, dimethyl sulfide, isopropanol, isobutanol, butan-2-ol or H₂/CO. Optimum growth of strain ZC-2T occurred in the presence of 0.2 M NaCl, pH 7.5-7.8 and temperature 50-55 °C with a specific growth rate of 0.084 h⁻¹. The mol% G+C content of the genomic DNA was 55.2 mol%. Based on these phenotypic and phylogenetic characteristics, strains ZC-2T, ZC-3 and ZC-6 are proposed to represent a novel species in the genus Methanoculleus and named Methanoculleus receptaculi sp. nov. The type strain is ZC-2T (CGMCC 1.5087T=DSM 18860T).

• Publisher: Oxford, UK : Blackwell Publishing Ltd,Blackwell Publishing Ltd,Blackwell,Oxford University Press
• Subject: Acetic acid / acetoclastic methanogenesis / Bacteriology / Biological and medical sciences / Carbon dioxide / China / Cocci / Deoxyribonucleic acid / Dimethyl sulfide / DNA / DNA, Bacterial - chemistry / DNA, Bacterial - genetics / Ethanol / Fundamental and applied biological sciences. Psychology / Gene sequencing / Growth rate / hydrogenotrophic methanogenesis / Isobutanol / isopropyl alcohol / methane production / Methanoculleus / Methanoculleus receptaculi / Methanogenesis / Methanogenic bacteria / methanogens / Methanomicrobiaceae - chemistry / Methanomicrobiaceae - classification / Methanomicrobiaceae - isolation & purification / Methanomicrobiaceae - physiology / Microbiology / Molecular Sequence Data / New species / nucleotide sequences / Oil and gas fields / Oil fields / oil reservoir / Petroleum - microbiology / Phylogeny / ribosomal RNA / rRNA 16S / sequence homology / Similarity / Sodium acetate / Sodium chloride / Sodium formate / specific growth rate / Substrates / Systematics / temperature / Trimethylamine / Water Microbiology