Journal Article

TOX3 regulates calcium-dependent transcription in neurons

Qiu, Zilong, Ghosh, Anirvan, Yuan, Shauna H

2009

Overview

We report the cloning and characterization of TOX3, a high mobility group box protein involved in mediating calcium-dependent transcription. TOX3 was identified as a calcium-dependent transactivator using the Transactivator Trap screen. We find that TOX3 interacts with both cAMP response element (CRE)-binding protein (CREB) and CREB-binding protein (CBP), and knockdown of the endogenous TOX3 by RNAi leads to significant reduction of calcium-induced c-fos expression and complete inhibition of calcium activation of the c-fos promoter. The effects of TOX3 on calcium-dependent transcription require the CRE elements. These observations identify TOX3 as an important regulator of calcium-dependent transcription and suggest that TOX3 exerts its effect on CRE-mediated transcription via its association with the CREB-CBP complex.

• Publisher: National Academy of Sciences,National Acad Sciences
• Subject: Animals / Antibodies / Base Sequence / Binding sites / Biological Sciences / Blotting, Northern / Calcium / Calcium - metabolism / Cell Line / Chloramphenicol O-Acetyltransferase - genetics / Cloning / CREB-Binding Protein - metabolism / cyclic AMP / Cyclic AMP Response Element-Binding Protein - metabolism / DNA / DNA Primers / Female / Gene expression / Gene expression regulation / Genes, fos / HEK293 cells / Humans / Immunohistochemistry / Molecular Sequence Data / Neurons / Neurons - metabolism / Pregnancy / Protein isoforms / Proteins / Rats / Rats, Long-Evans / Ribonucleic acid / RNA / RNA interference / Transactivators / Transcription, Genetic - physiology / Transcriptional regulatory elements