Journal Article

Adenovirus-mediated gene expression imaging to directly detect sentinel lymph node metastasis of prostate cancer

Burton, Jeremy B, Mulholland, David J, Koh, Sok Boon S, Chatziioannou, Arion F, Johnson, Mai, Wu, Lily, Phelps, Michael E, Stout, David, Wu, Hong, Sato, Makoto

2008

Overview

The degree of lymph-node metastasis in prostate cancer is crucial for both staging the disease and planning treatment. Here, Burton and colleagues describe a one-step, non-invasive imaging technology using prostate-specific adenoviral vectors that express imaging reporter genes. This set-up specifically and accurately detects lymph-node metastases in a model of human prostate cancer and eliminates the need for invasive lymphadenectomy required by the current lymphoscintigraphy method. The accurate assessment of nodal involvement in prostate cancer is crucial to planning treatment, yet there is a shortage of noninvasive imaging techniques capable of visualizing nodal lesions directly. This study demonstrates the feasibility of using recombinant human adenoviral vectors to detect nodal metastases in a human prostate cancer model. This was achieved by the prostate-restricted expression of optical and positron emission tomography (PET) imaging reporter genes by the viral vector coupled with the innate lymphotropic properties of adenovirus. We show that peritumoral administration of these vectors results in the direct detection of reporter gene expression in metastatic lesions within sentinel lymph nodes. Notably, this approach parallels the current lymphoscintigraphy method but enables the direct PET visualization of sentinel lymph node metastases, eliminating the need for invasive lymphadenectomy. These findings may lead to more effective diagnostic and therapeutic strategies for individuals with advanced-stage prostate cancer.

• Publisher: Nature Publishing Group US,Nature Publishing Group
• Subject: Adenoviridae - genetics / Adenoviridae - metabolism / Adenovirus / Adenoviruses / Animals / Biomedical and Life Sciences / Biomedicine / Cancer Research / Diagnostic tests / Emissions / Gene expression / Gene Expression Regulation, Viral / Genetic aspects / Genetic recombination / Humans / Indoles / Infectious Diseases / Lesions / Lymph nodes / Lymph Nodes - pathology / Lymphatic Metastasis / Magnetic resonance imaging / Male / Medical imaging / Metabolic Diseases / Metastasis / Mice / Mice, Transgenic / Molecular Medicine / Neoplasm Metastasis / Neoplasm Transplantation / Neurosciences / Positron-Emission Tomography - methods / Prostate cancer / Prostatic Neoplasms - genetics / Prostatic Neoplasms - metabolism / technical-report / Tomography / Tomography, X-Ray Computed - methods / Viruses