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In-situ-localisation of nitrate reductase in maize roots
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In-situ-localisation of nitrate reductase in maize roots
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Journal Article
In-situ-localisation of nitrate reductase in maize roots
1994
Overview
The localization of nitrate reductase (NR; EC 1.6.6.2) in cells of root tissues of Zea mays L. (W64A W182L) was determined using post-embedding immunogold labeling at the electron-microscopy level and using silver enhancement of the colloidal-gold signal for light microscopy. Nitrate reductase is located in the cytoplasm of root epidermal and cortical cells, and in the cells of the parenchyma and pericycle within the vascular cylinder. A weaker signal was also obtained in parenchymal cells of the pith lying next to the xylem. A positive signal for NR protein was seen in the chloroplast fraction of maize leaves and in the plastid fraction of roots. This signal was lost when affinity-purified antibodies were used. Sections of Lowicryl-embedded tissue were found to be suitable for the localization of the non-abundant NR protein when adequate controls and signal-enhancement procedures were used.
Publisher
Springer-Verlag,Springer
Subject
