Asset Details
Do you wish to reserve the book?
Models for the architecture of the human inner kinetochore on centromeric α-satellite CENP-A nucleosome arrays
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Models for the architecture of the human inner kinetochore on centromeric α-satellite CENP-A nucleosome arrays
Do you wish to request the book?
Models for the architecture of the human inner kinetochore on centromeric α-satellite CENP-A nucleosome arrays
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Models for the architecture of the human inner kinetochore on centromeric α-satellite CENP-A nucleosome arrays
2026
Overview
Human kinetochores assemble onto centromeric DNA comprising repetitive arrays of the 171 bp α-satellite sequence. To understand the higher-order architecture of the inner kinetochore assembled onto α-satellite arrays, we show cryo-EM structures of CCAN with free DNA, and α-satellite repeat monomers and dimers with CENP-A nucleosomes. CCAN bound to free DNA and a monomeric CENP-A nucleosome engages 70 bp of DNA comprising 30 bp of an upstream α-satellite repeat. This upstream DNA interacts with the histone-fold domain subunits of the CENP-TWSX module in a manner resembling how nucleosomes wrap DNA gyres. A complex of CCAN assembled onto a dimeric α-satellite repeat with two CENP-A nucleosomes shows that CCAN can only be accommodated on the linker DNA by unwrapping DNA from both the CENP-TWSX module and the upstream nucleosome. We discuss the implications of these results for models of CCAN assembly on arrays of α-satellite chromatin containing CENP-A nucleosomes.
Based on cryo-EM structures, here the authors propose three models for how the inner kinetochore and CENP-A nucleosomes are organized on repetitive alpha-satellite sequences, providing insights into the higher-order architecture of human centromeres.
Publisher
Nature Publishing Group UK,Nature Publishing Group
Subject
/ 82/80
/ 82/83
/ Arrays
/ Centromere Protein A - chemistry
/ Centromere Protein A - genetics
/ Centromere Protein A - metabolism
/ Centromere Protein A - ultrastructure
/ Chromosomal Proteins, Non-Histone - metabolism
/ DNA
/ Gyres
/ Histones
/ Humanities and Social Sciences
/ Humans
/ Kinetochores - ultrastructure
/ Modules
/ Nucleosomes - ultrastructure
/ Science
