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New culture medium from Lentils (Lens exculenta) seeds for growth Leishmania parasite and some bacteria and fungi
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New culture medium from Lentils (Lens exculenta) seeds for growth Leishmania parasite and some bacteria and fungi
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Journal Article
New culture medium from Lentils (Lens exculenta) seeds for growth Leishmania parasite and some bacteria and fungi
2021
Overview
The Lentils ( Lens exculenta ) were used in research of a new culture medium aimed at the growth of leishmania parasite in vitro. The medium was composed of two phases. The Lens exculenta was used in preparation (with or without added misshapen blood). In the liquid phase, dextrolite solution was used as an oral perfusion solution, instead of lock solution. This study showed increased in numbers of promasitgote in new culture media and this The study showed an increase in the growth of the shape in the center of the plant, and that this increase and the number of parasites was a significant increase compared to the center of NNN-media. The average number of parasites on the eighth day of growth, which represents the peak growth in these groups (2425 and 2650) ×10 4 cell/ml, respectively. The growth of the parasite continued but at a lower rate and good viability until the twentieth day the number of parasites reached to (500 and 466.6) ×10 4 cell/ml, respectively. This study showed that the percentage of the parasite viability was good and increased from the second day to the highest on the eighth day (96 % and 95%) respectively. On the 20 th day, it was only (30% and 15 %) respectively. Blood added to the new medium receives good growth but lasts only 14 days with no subculture.
