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Enhanced steroidal alkaloids accumulation under abiotic elicitation of in vitro cultures of Fritillaria roylei using response surface methodology
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Enhanced steroidal alkaloids accumulation under abiotic elicitation of in vitro cultures of Fritillaria roylei using response surface methodology
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Journal Article
Enhanced steroidal alkaloids accumulation under abiotic elicitation of in vitro cultures of Fritillaria roylei using response surface methodology
2024
Overview
Fritillaria roylei
is an endangered bulbous perennial plant with high ethnopharmacological value. The high morphophysiological dormancy, habitat destruction, and irresponsive collection have resulted in the endangered status of
F. roylei
plants. The present study established an efficient
in vitro
regeneration and multiplication protocol for bulb multiplication. Gamborg’s B5 medium supplemented with NAA (5.3 µM), BAP (4.4 µM) and kinetin (4.6 µM), spermidine (137 µM), and NLN vitamin mixture (1 mg/mL) was used efficiently for bulb multiplication. Each bulb produced 10–12 bulblets after 12–14 wk showing clonal fidelity under standardized
in vitro
conditions. The phytochemical analysis of the regenerated bulbs detected four important steroidal alkaloids, namely, solanidine, peimisine, petisidine, and fritilline A. Elicitation (jasmonic acid, methyl jasmonate, salicylic acid, and gibberellic acid) of regenerated bulbs using response surface methodology demonstrated higher accumulation of solanidine (2.6 folds), and peimisine, petisidine and fritilline A (up to 1.4 folds each). The study will facilitate mass multiplication and conservation of endangered species of medicinal importance and enable understanding the biosynthesis of bioactives in the species.
Publisher
Springer US,Springer Nature B.V
