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DNA barcoding and genetic distances in three genera of Naididae (Annelida: Clitellata)
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DNA barcoding and genetic distances in three genera of Naididae (Annelida: Clitellata)
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DNA barcoding and genetic distances in three genera of Naididae (Annelida: Clitellata)
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Journal Article
DNA barcoding and genetic distances in three genera of Naididae (Annelida: Clitellata)
2024
Overview
Identification of organism groups such as Oligochaetes to species level is difficult to achieve using morphological examination alone. This is especially true in species where sexual reproduction is rare, such as the subfamily Naidedae. Here, variation in the barcoding marker COI of three genera of freshwater clitellates,
Dero
,
Nais
, and
Pristina
(Clitellata: Naididae) was studied, using publicly available sequences together with newly generated ones, with the aim to test for the presence of global barcode gaps. In total 17 sequences of
Dero
spp., 168 of
Nais
spp., and 22 sequences of
Pristina
spp. were included in the analyses. The uncorrected pairwise distances in the
Dero
dataset ranges from 0.00 to 0.18, in the
Nais
dataset they range from 0.00 to 0.21, and in the
Pristina
dataset they range from 0.00 to 0.36, with a large gap between 0.21 and 0.35. No global barcoding gap was found in any of the datasets. In all three genera clusters including more than one species and/or species found in more than one cluster were found, indicating taxonomical problems, such as cryptic species and misidentified sequences.
