Asset Details
Do you wish to reserve the book?
Autophagic Response of the Liver Cells to Schistosoma mansoni Infection and Praziquantel Treatment
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Autophagic Response of the Liver Cells to Schistosoma mansoni Infection and Praziquantel Treatment
Do you wish to request the book?
Autophagic Response of the Liver Cells to Schistosoma mansoni Infection and Praziquantel Treatment
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Autophagic Response of the Liver Cells to Schistosoma mansoni Infection and Praziquantel Treatment
2025
Overview
Purpose
The autophagy process is critical for cell survival, homeostasis, and functions. Autophagy deregulation was linked to several liver diseases; however, little is known about autophagy status during schistosomiasis and praziquantel (PZQ) treatment. This study is exploring autophagy status during schistosomiasis and praziquantel treatment in the hepatocytes, macrophages, and hepatic stellate cells (HSCs) in mice, and linking the variations in these levels to the parasitic parameters.
Methods
CD-1 Swiss female albino mice were allocated into three groups: negative control,
Schistosoma mansoni
(
S. mansoni
)-infected, and the third group was
S. mansoni-
infected and treated with PZQ. Liver sections were stained for autophagy markers (LC3 and P62) by immunohistochemistry.
Results
At 12 weeks post infection, P62 increased in
S. mansoni
-infected mice in hepatocytes, macrophages, and HSCs compared to negative controls (
P
= 0.038,
P
< 0.001,
P
= 0.004, respectively) and decreased in PZQ-treated group compared to
S. mansoni
-infected mice in hepatocytes and macrophages only (
P
< 0.001,
P
= 0.004, respectively). LC3 displayed higher levels in
S. mansoni-
infected mice compared to negative controls in hepatocytes, macrophages, and HSCs (
P
= 0.01,
P
< 0.001,
P
< 0.001, respectively), while in PZQ-treated group, it decreased in macrophages and HSCs compared to
S. mansoni
-infected mice (
P
= 0.016,
P
< 0.001, respectively). Correlations were found between the number of eggs and autophagy status, mostly in HSCs.
Conclusion
Schistosomiasis inhibits autophagy in hepatocytes, macrophages, and HSCs, while PZQ treatment reverses autophagy levels in hepatocytes and macrophages, but not in HSCs. More research is needed to understand the autophagy behavior in HSCs during different stages of schistosomiasis to promote development of autophagy-based anti-fibrotic drugs.
Publisher
Springer International Publishing,Springer Nature B.V
Subject
Animal Systematics/Taxonomy/Biogeography
/ Animals
/ Anthelmintics - administration & dosage
/ Anthelmintics - therapeutic use
/ Biomedical and Life Sciences
/ Ecology
/ Female
/ Hepatic Stellate Cells - drug effects
/ Hepatic Stellate Cells - parasitology
/ Liver
/ Mice
/ Praziquantel - administration & dosage
/ Praziquantel - therapeutic use
/ Proteins
/ Schistosoma mansoni - drug effects
/ Schistosoma mansoni - physiology
/ Schistosomiasis mansoni - drug therapy
/ Schistosomiasis mansoni - parasitology
/ Schistosomiasis mansoni - pathology
/ Software
/ Tissues
