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Surfactin and its Antibacterial Mechanism on Staphylococcus Aureus and Application in Pork Preservation
by
Mintah, Benjamin Kumah
, He, Ronghai
, Yin, Xiulian
, Yan, Pengfei
, Shu, Zhenzhen
, Ma, Haile
, Dai, Chunhua
in
Agriculture
/ antibacterial properties
/ Antiinfectives and antibacterials
/ Bacillus subtilis
/ biochemical pathways
/ bioprocessing
/ Biotechnology
/ Cell membranes
/ Cell walls
/ Chemistry
/ Chemistry and Materials Science
/ Chemistry/Food Science
/ chlorides
/ Congeners
/ deformation
/ electron microscopy
/ Enzymatic activity
/ Enzyme activity
/ Enzymes
/ Food Science
/ Membrane potential
/ Metabolism
/ minimum inhibitory concentration
/ Nucleic acids
/ Pork
/ Preservation
/ Scanning electron microscopy
/ soybean meal
/ Soybeans
/ Staphylococcus aureus
/ Surfactin
/ texture
2025
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Surfactin and its Antibacterial Mechanism on Staphylococcus Aureus and Application in Pork Preservation
by
Mintah, Benjamin Kumah
, He, Ronghai
, Yin, Xiulian
, Yan, Pengfei
, Shu, Zhenzhen
, Ma, Haile
, Dai, Chunhua
in
Agriculture
/ antibacterial properties
/ Antiinfectives and antibacterials
/ Bacillus subtilis
/ biochemical pathways
/ bioprocessing
/ Biotechnology
/ Cell membranes
/ Cell walls
/ Chemistry
/ Chemistry and Materials Science
/ Chemistry/Food Science
/ chlorides
/ Congeners
/ deformation
/ electron microscopy
/ Enzymatic activity
/ Enzyme activity
/ Enzymes
/ Food Science
/ Membrane potential
/ Metabolism
/ minimum inhibitory concentration
/ Nucleic acids
/ Pork
/ Preservation
/ Scanning electron microscopy
/ soybean meal
/ Soybeans
/ Staphylococcus aureus
/ Surfactin
/ texture
2025
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Surfactin and its Antibacterial Mechanism on Staphylococcus Aureus and Application in Pork Preservation
by
Mintah, Benjamin Kumah
, He, Ronghai
, Yin, Xiulian
, Yan, Pengfei
, Shu, Zhenzhen
, Ma, Haile
, Dai, Chunhua
in
Agriculture
/ antibacterial properties
/ Antiinfectives and antibacterials
/ Bacillus subtilis
/ biochemical pathways
/ bioprocessing
/ Biotechnology
/ Cell membranes
/ Cell walls
/ Chemistry
/ Chemistry and Materials Science
/ Chemistry/Food Science
/ chlorides
/ Congeners
/ deformation
/ electron microscopy
/ Enzymatic activity
/ Enzyme activity
/ Enzymes
/ Food Science
/ Membrane potential
/ Metabolism
/ minimum inhibitory concentration
/ Nucleic acids
/ Pork
/ Preservation
/ Scanning electron microscopy
/ soybean meal
/ Soybeans
/ Staphylococcus aureus
/ Surfactin
/ texture
2025
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Surfactin and its Antibacterial Mechanism on Staphylococcus Aureus and Application in Pork Preservation
Journal Article
Surfactin and its Antibacterial Mechanism on Staphylococcus Aureus and Application in Pork Preservation
2025
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Overview
This study investigated the antibacterial capacity and mechanism of surfactin (prepared by fermenting soybean meal with
Bacillus subtilis
SOPC5) against
Staphylococcus aureus
by examining its influences on the bacterial morphology, cell wall and membrane, as well as metabolic activity. The efficiency of surfactin in pork preservation was also investigated. HPLC-MS analysis showed that surfactin’s purity reached 97.4%, containing five congeners. The minimal inhibition concentration (MIC) and minimal bactericidal concentration (MBC) of surfactin against
S. aureus
were 1.6 and 3.2 mg/mL, respectively. The result of scanning electron microscopy observation revealed that 4 h of surfactin exposure resulted in deformation and even collapse of
S. aureus
. AKP assay, membrane potential analysis, extracellular protein and nucleic acid content measurement, and PI staining intensity assay showed that surfactin destroyed the cell wall and cell membrane of
S. aureus.
Decreases in iodonitrotetrazolium chloride levels and ATP enzyme activity indicated that surfactin could inhibit the metabolic ability of
S. aureus
. Furthermore, surfactin demonstrated an excellent antibacterial action on
S. aureus
within 5 days of pork storage without changing its texture. These findings suggest that sufactin has a great application potential in pork preservation.
Graphical Abstract
Publisher
Springer US,Springer Nature B.V
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