Asset Details
Do you wish to reserve the book?
Morphological and molecular characterization of two species of genus Ageratum
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Morphological and molecular characterization of two species of genus Ageratum
Do you wish to request the book?
Morphological and molecular characterization of two species of genus Ageratum
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Morphological and molecular characterization of two species of genus Ageratum
2024
Overview
Background
The species of genus
Ageratum
(family Asteraceae) are distributed in various parts of the world.
Ageratum conyzoides
and
A. houstonianum
are the most commonly occurring species in India. These species are quite similar in their morphology thus creating a challenge in identification during the field survey and taxonomic validation. The accurate identification of the species is highly significant especially when those are of medicinal interest. To overcome the barriers in morphological based identification, DNA barcoding has been employed during the present investigation.
Methods and results
Morphological and DNA barcodes matK and ITS genes, were employed to differentiate between
Ageratum conyzoides
and
A. houstonianum
. The obtained matK and ITS gene sequences were submitted to GenBank and BOLD system to obtain accession numbers. The DNA sequences were aligned with database sequences using BLAST and phylogenetic trees were constructed through neighbor-joining algorithm in MEGA 11 software. The distinguish features of
A. conyzoides
include ovate to elliptic-oblong leaves with a cuneate base and inflorescence heads forming domed to flat-topped clusters. However,
A. houstonianum
has triangular to ovate leaves with a cordate to truncate base, cymose clusters in the inflorescence and stipulate glandular involucre bracts. The matK gene has shown the highest identity percentages (100%) for
A. houstonianum
and 99.87% for
A. conyzoides
. The phylogenetic tree analysis has demonstrated a close association of
A. conyzoides
and
A. houstonianum
with their respective species, supported by bootstrap values in the matK and ITS trees.
Conclusion
This study revealed that morphological and molecular data can be successfully utilized in the identification of
A. conyzoides
and
A. houstonianum
. The matK and ITS barcodes provide promising results in the identification of
Ageratum
species, with their phylogeny supporting classification within the family asteraceae.
Publisher
Springer Netherlands,Springer Nature B.V
Subject
