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Development of novel polymorphic microsatellite markers for Picea brachytyla
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Development of novel polymorphic microsatellite markers for Picea brachytyla
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Development of novel polymorphic microsatellite markers for Picea brachytyla
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Journal Article
Development of novel polymorphic microsatellite markers for Picea brachytyla
2022
Overview
Background
Picea brachytyla
is a unique tree species in China. Due to being extensively exploited in the past, it is listed as Vulnerable in the IUCN Red List. It is mainly distributed across the Hengduan and Daba-Qinglin mountains and has been found in other areas including Sichuan Province and Qinghai Province, China. Microsatellites, or simple sequence repeats (SSRs), are widely used in correlational studies of genetic protection. Few markers have been developed for
P. brachytyla
because of the small number of trees and scholarly resources available for study.
Methods and Results
The genomic DNA of
P. brachytyla
was sequenced using the DNBSEQ platform, and unigenes were obtained after assembly and deredundancy. Of the 100 primer pairs screened, we isolated 10 useful microsatellite loci from
P. brachytyla
genes. The observed and expected heterozygosity values ranged from 0.173 (P24) to 0.788 (P79; mean 0.469) and 0.199 (P87) to 0.911 (P79; mean 0.700), respectively. Polymorphism-information content (PIC) ranged from 0.190 (P84) to 0.904 (P79; mean 0.666). Only P84 and P72 were in a Hardy-Weinberg equilibrium (
P
> 0.05) in the different
P. brachytyla
populations. All the levels of linkage disequilibrium (LD) were high for the 10 SSR loci indicating that there were no autocorrelations among the 10 SSR loci.
Conclusions
The novel polymorphic microsatellite markers showed high polymorphism for
P. brachytyla
. These polymorphic microsatellites can provide a basis for future conservation and genetic research on this rare plant species.
