Journal Article

Tumor-Infiltrating NETs Predict Postsurgical Survival in Patients with Pancreatic Ductal Adenocarcinoma

Gao, He-Li, Li, Hao, Li, Shuo, Liu, Liang, Wang, Wen-Quan, Jin, Wei, Xu, Hua-Xiang, Zhang, Shi-Rong, Yu, Xian-Jun, Wu, Chun-Tao, Xu, Jin-Zhi, Ni, Quan-Xing, Qi, Zi-Hao

2019

Overview

Background Tumor-infiltrating neutrophils (TINs) indicate poor prognosis for patients with pancreatic ductal adenocarcinoma (PDAC). Activated neutrophils can generate neutrophil extracellular traps (NETs). Little is known about the presence and prognostic significance of tumor-infiltrating NETs in PDAC. Methods This study enrolled 317 patients, in two independent sets (training and validation), who underwent curative pancreatectomy for PDAC in Shanghai Cancer Center. TINs and NETs were identified by immunohistochemical staining for CD15 and citrullinated histone H3, respectively. The relationship between clinicopathological features and outcomes was analyzed. Accuracy of prognostic prediction models was evaluated using concordance index (C-index) and Akaike information criterion (AIC). Results NETs were associated with OS (both, P  < 0.001) and RFS (both, P  < 0.001) in the training and validation sets. Tumor-infiltrating NETs predicted poor postsurgical survival of patients with PDAC. Moreover, multivariate analysis identified NETs and AJCC TNM stage as two independent prognostic factors for OS and RFS. Combination of NETs with the 8th edition TNM staging system (C-index, 0.6994 and 0.6669, respectively; AIC, 1067 and 1126, respectively) generated a novel model that improved the predictive accuracy for survival in both sets (C-index, 0.7254 and 0.7117, respectively; AIC, 1047 and 1102, respectively). The model combining presence of NETs with the 7th edition AJCC TNM staging system also had improved predictive accuracy. Conclusions NETs were an independent prognostic factor in PDAC and incorporation of NETs along with the standard TNM stating system refined risk-stratification and predicted survival in PDAC with improved accuracy.

• Publisher: Springer International Publishing,Springer Nature B.V
• Subject: Accuracy / Adenocarcinoma / Carcinoma, Pancreatic Ductal - mortality / Carcinoma, Pancreatic Ductal - pathology / Carcinoma, Pancreatic Ductal - surgery / Citrulline / Cohort Studies / Extracellular Traps / Female / Follow-Up Studies / Histone H3 / Humans / Leukocytes (neutrophilic) / Male / Medical prognosis / Medicine / Medicine & Public Health / Middle Aged / Multivariate analysis / Neutrophils / Oncology / Pancreas / Pancreatectomy - mortality / Pancreatic cancer / Pancreatic Neoplasms / Pancreatic Neoplasms - mortality / Pancreatic Neoplasms - pathology / Pancreatic Neoplasms - surgery / Pancreatic Tumors / Prognosis / Surgery / Surgical Oncology / Survival Rate