Asset Details
Do you wish to reserve the book?
Chance and pleiotropy dominate genetic diversity in complex bacterial environments
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Chance and pleiotropy dominate genetic diversity in complex bacterial environments
Do you wish to request the book?
Chance and pleiotropy dominate genetic diversity in complex bacterial environments
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Chance and pleiotropy dominate genetic diversity in complex bacterial environments
2019
Overview
How does environmental complexity affect the evolution of single genes? Here, we measured the effects of a set of
Bacillus subtilis
glutamate dehydrogenase mutants across 19 different environments—from phenotypically homogeneous single-cell populations in liquid media to heterogeneous biofilms, plant roots and soil populations. The effects of individual gene mutations on organismal fitness were highly reproducible in liquid cultures. However, 84% of the tested alleles showed opposing fitness effects under different growth conditions (sign environmental pleiotropy). In colony biofilms and soil samples, different alleles dominated in parallel replica experiments. Accordingly, we found that in these heterogeneous cell populations the fate of mutations was dictated by a combination of selection and drift. The latter relates to programmed prophage excisions that occurred during biofilm development. Overall, for each condition, a wide range of glutamate dehydrogenase mutations persisted and sometimes fixated as a result of the combined action of selection, pleiotropy and chance. However, over longer periods and in multiple environments, nearly all of this diversity would be lost—across all the environments and conditions that we tested, the wild type was the fittest allele.
The study of the impact of mutations in the glutamate dehydrogenase enzyme from
Bacillus subtilis
across multiple settings, including liquid cultures, biofilms and in soil, highlights how bacterial growth states and the environment can differentially impact protein evolution.
Publisher
Nature Publishing Group UK,Nature Publishing Group
