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aChIP is an efficient and sensitive ChIP-seq technique for economically important plant organs
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aChIP is an efficient and sensitive ChIP-seq technique for economically important plant organs
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Journal Article
aChIP is an efficient and sensitive ChIP-seq technique for economically important plant organs
2024
Overview
Chromatin immunoprecipitation followed by sequencing (ChIP-seq) is crucial for profiling histone modifications and transcription factor binding throughout the genome. However, its application in economically important plant organs (EIPOs) such as seeds, fruits and flowers is challenging due to their sturdy cell walls and complex constituents. Here we present advanced ChIP (aChIP), an optimized method that efficiently isolates chromatin from plant tissues while simultaneously removing cell walls and cellular constituents. aChIP precisely profiles histone modifications in all 14 tested EIPOs and identifies transcription factor and chromatin-modifying enzyme binding sites. In addition, aChIP enhances ChIP efficiency, revealing numerous novel modified sites compared with previous methods in vegetative tissues. aChIP reveals the histone modification landscape for rapeseed dry seeds, highlighting the intricate roles of chromatin dynamics during seed dormancy and germination. Altogether, aChIP is a powerful, efficient and sensitive approach for comprehensive chromatin profiling in virtually all plant tissues, especially in EIPOs.This study presents an advanced ChIP-seq method, provides a valuable epigenomics resource for 14 economically important plant organs and reveals histone modification landscape dynamics and functions during rapeseed seed dormancy and germination.
Publisher
Nature Publishing Group
Subject
