Journal Article

Activation of the GPX4/TLR4 Signaling Pathway Participates in the Alleviation of Selenium Yeast on Deltamethrin-Provoked Cerebrum Injury in Quails

Zhang, Zhigang, Li, Siyu, Wang, Xiaoqiao, Lv, Yueying, Wu, Pengfei, Yu, Zhongxian, Yang, Qingyue, Qu, Bing, Li, Jiayi, Liao, Yuge, Han, Bing

2022

Overview

Deltamethrin (DLM) is a member of pyrethroid pesticide widely applied for agriculture and aquaculture, and its residue in the environment seriously threatens the bio-safety. The cerebrum might be vulnerable to pesticide-triggered oxidative stress. However, there is no specific antidote for treating DLM-triggered cerebral injury. Selenium (Se) is an essential trace element functionally forming selenoprotein glutathione peroxidase (GPX) in antioxidant defense. Se yeast (SY) is a common and effective organic form of Se supplement with high selenomethionine content. Accordingly, this study focused on investigating the therapeutic potential of SY on DLM-induced cerebral injury in quails after chronically exposing to DLM and exploring the underlying mechanisms. Quails were treated with/without SY (0.4 mg kg −1 SY added in standard diet) in the presence/absence of DLM (45 mg kg −1 body weight intragastrically) for 12 weeks. The results showed SY supplementation ameliorated DLM-induced cerebral toxicity. Concretely, SY elevated the content of Se and increased GPX4 level in DLM-treated quail cerebrum. Furthermore, SY enhanced antioxidant defense system by upregulating nuclear factor-erythroid-2-related factor 2 (Nrf2) associated members. Inversely, SY diminished the changes of apoptosis- and inflammation-associated proteins and genes including toll-like receptor 4 (TLR4). Collectively, our results suggest that dietary SY protects against DLM-induced cerebral toxicity in quails via positively regulating the GPX4/TLR4 signaling pathway. GPX4 may be a potential therapeutic target for insecticide-induced biotoxicity.

• Publisher: Springer US,Springer Nature B.V
• Subject: Animals / Antioxidants / Antioxidants - metabolism / Apoptosis / Biomedical and Life Sciences / Biomedicine / Body weight / Cell Biology / Cerebrum / Cerebrum - metabolism / Deltamethrin / Glutathione peroxidase / Homeostasis / Laboratory animals / Neurobiology / Neurology / Neurosciences / Nitriles / Organisms / Oxidative stress / Pesticides / Proteins / Pyrethrins / Pyrethroids / Quail - metabolism / Saccharomyces cerevisiae - metabolism / Selenium / Selenium - pharmacology / Selenomethionine / Signal Transduction / Therapeutic targets / TLR4 protein / Toll-Like Receptor 4 - metabolism / Toll-like receptors / Toxicity / Trace elements (nutrients) / Tumor necrosis factor-TNF